Response Surface Methodology-Genetic Algorithm Based Medium Optimization, Purification, and Characterization of Cholesterol Oxidase from Streptomyces rimosus

The applicability of the statistical tools coupled with artificial intelligence techniques was tested to optimize the critical medium components for the production of extracellular cholesterol oxidase (COD; an enzyme of commercial interest) from Streptomyces rimosus MTCC 10792. The initial medium co...

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Bibliographic Details
Published in:Scientific reports 2018-07, Vol.8 (1), p.10913-13, Article 10913
Main Authors: Srivastava, Akanksha, Singh, Vineeta, Haque, Shafiul, Pandey, Smriti, Mishra, Manisha, Jawed, Arshad, Shukla, P. K., Singh, P. K., Tripathi, C. K. M.
Format: Article
Language:English
Subjects:
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Algorithms
Ammonium
Artificial intelligence
Cholesterol
Cholesterol oxidase
Dextrose
Enzymes
Forest soils
Genetic algorithms
Humanities and Social Sciences
Mathematical models
multidisciplinary
Purification
Science
Science (multidisciplinary)
Starch
Statistical analysis
Statistical models
Streptomyces rimosus
Yeasts
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Summary:The applicability of the statistical tools coupled with artificial intelligence techniques was tested to optimize the critical medium components for the production of extracellular cholesterol oxidase (COD; an enzyme of commercial interest) from Streptomyces rimosus MTCC 10792. The initial medium component screening was performed using Placket-Burman design with yeast extract, dextrose, starch and ammonium carbonate as significant factors. Response surface methodology (RSM) was attempted to develop a statistical model with a significant coefficient of determination (R 2  = 0.89847), followed by model optimization using Genetic Algorithm (GA). RSM-GA based optimization approach predicted that the combination of yeast extract, dextrose, starch and ammonium carbonate at concentrations 0.99, 0.8, 0.1, and 0.05 g/100 ml respectively, has resulted in 3.6 folds increase in COD production (5.41 U/ml) in comparison with the un-optimized medium (1.5 U/ml). COD was purified 10.34 folds having specific activity of 12.37 U/mg with molecular mass of 54 kDa. The enzyme was stable at pH 7.0 and 40 °C temperature. The apparent Michaelis constant (K m ) and V max values of COD were 0.043 mM and 2.21 μmol/min/mg, respectively. This is the first communication reporting RSM-GA based medium optimization, purification and characterization of COD by S. rimosus isolated from the forest soil of eastern India.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-29241-9