Cell paintballing using optically targeted coacervate microdroplets

We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of ste...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2015-11, Vol.6 (11), p.6106-6111
Main Authors: Armstrong, James P K, Olof, Sam N, Jakimowicz, Monika D, Hollander, Anthony P, Mann, Stephen, Davis, Sean A, Miles, Mervyn J, Patil, Avinash J, Perriman, Adam W
Format: Article
Language:English
Subjects:
Affinity
Chemistry
Dyes
Membranes
Oligonucleotides
Payloads
Stem cells
Stimulation
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Summary:We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of stem cell membranes using a dynamic holographic assembler, resulting in spontaneous coacervate microdroplet-membrane fusion. The facile preparation, high sequestration efficiency and inherent membrane affinity of the microdroplets make this novel "cell paintballing" technology a highly advantageous option for spatially-directed cell functionalization, with potential applications in single cell stimulation, transfection and differentiation.
ISSN:2041-6520
2041-6539
DOI:10.1039/c5sc02266e