Arabidopsis thaliana and Pseudomonas Pathogens Exhibit Stable Associations over Evolutionary Timescales

• Published in: Cell host & microbe 2018-07, Vol.24 (1), p.168-179.e4
• Main Authors: Karasov, Talia L., Almario, Juliana, Friedemann, Claudia, Ding, Wei, Giolai, Michael, Heavens, Darren, Kersten, Sonja, Lundberg, Derek S., Neumann, Manuela, Regalado, Julian, Neher, Richard A., Kemen, Eric, Weigel, Detlef
• Format: Article
• Language: English
• Subjects: A. thaliana; Arabidopsis - microbiology; Biological Evolution; clonal expansion; Crops, Agricultural - microbiology; DNA, Bacterial - genetics; Life Sciences; Metagenome; microbial population genomics; pathogenicity; Phylogeny; Plant Diseases - microbiology; Plant Leaves - microbiology; pseudomonas; Pseudomonas - genetics; Pseudomonas - pathogenicity; Pseudomonas Infections - microbiology; RNA, Ribosomal, 16S - genetics; Whole Genome Sequencing
• ISSN: 1931-3128; 1934-6069
• DOI: 10.1016/j.chom.2018.06.011

Crop disease outbreaks are often associated with clonal expansions of single pathogenic lineages. To determine whether similar boom-and-bust scenarios hold for wild pathosystems, we carried out a multi-year, multi-site survey of Pseudomonas in its natural host Arabidopsis thaliana. The most common Pseudomonas lineage corresponded to a ubiquitous pathogenic clade. Sequencing of 1,524 genomes revealed this lineage to have diversified approximately 300,000 years ago, containing dozens of genetically identifiable pathogenic sublineages. There is differentiation at the level of both gene content and disease phenotype, although the differentiation may not provide fitness advantages to specific sublineages. The coexistence of sublineages indicates that in contrast to crop systems, no single strain has been able to overtake the studied A. thaliana populations in the recent past. Our results suggest that selective pressures acting on a plant pathogen in wild hosts are likely to be much more complex than those in agricultural systems. [Display omitted] •Wild A. thaliana is regularly colonized by a single Pseudomonas OTU•Strains within this OTU diverged from one another at least 300,000 years ago•Many strains can cause disease and are classified as pathogenic•In contrast to agriculture, no single pathogenic strain dominates host populations Disease outbreaks in agriculture are often associated with clonal expansions of single pathogenic lineages. In this study, Karasov et al. show that in populations of a wild plant, no single lineage of an abundant pathogen takes over the host population. Genetic and species diversity may prevent clonal expansions in nature.