MRI Guiding of the Middle Cerebral Artery Occlusion in Rats Aimed to Improve Stroke Modeling

• Published in: Translational stroke research 2018-08, Vol.9 (4), p.417-425
• Main Authors: Gubskiy, Ilya L., Namestnikova, Daria D., Cherkashova, Elvira A., Chekhonin, Vladimir P., Baklaushev, Vladimir P., Gubsky, Leonid V., Yarygin, Konstantin N.
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Cardiology; Carotid arteries; Disease Models, Animal; Doppler effect; Infarction, Middle Cerebral Artery - complications; Ischemia; Magnetic Resonance Imaging; Male; Medical imaging; Neurology; Neurosciences; Neurosurgery; Original; Original Article; Rats; Rats, Wistar; Stroke - diagnostic imaging; Stroke - etiology; Surgery; Surgical outcomes; Sutures; Thyroid gland; Vascular Surgery; Veins & arteries
• ISSN: 1868-4483; 1868-601X
• DOI: 10.1007/s12975-017-0590-y

The middle cerebral artery occlusion (MCAO) model in rats closely imitates ischemic stroke and is widely used. Existing instrumental methods provide a certain level of MCAO guidance, but monitoring of the MCA-occluding intraluminal filament position and possible complications can be improved. The goal of this study was to develop a MRI-based method of simultaneous control of the filament position, blood flow in the intracranial vessels, and hemorrhagic complications. Rats were subjected to either MRI-guided MCAO (group 1, n  = 51) or MCAO without MRI control (group 2, n  = 38). After operation, group 1 rats were transferred into a MRI scanner for the control of the filament position and possible complications. Ninety minutes after the onset of MCAO, the filament was removed in rats of both groups and MRI control of the infarct volume and hemorrhagic complications performed. High-resolution T1- and T2-weighted imaging performed immediately after filament insertion provided visualization of the filament position, blood flow in brain arteries, and complications related to inappropriate filament insertion. It permitted replacement of wrongly positioned filaments and exclusion of animals with complications from the experiment. MRI-based MCAO guiding provided real-time intra-operational monitoring of crucial parameters determining MCAO suitability for stroke modeling, including better assessment of the operation outcomes in individual animals and significant enhancement of the model success rate. The possibility of simultaneous visualization of the filament, blood flow in the arteries, brain tissue, and hemorrhagic complications is the principal advantage of the proposed method over other instrumental methods of MCAO quality control. Graphical Abstract MRI-guided middle cerebral artery occlusion technique permits intra-operational monitoring via direct non-invasive simultaneous visualization of the filament, blood flow in the arteries, brain tissue, and hemorrhagic complications. It provides better assessment of MCAO outcomes in individual animals and significant enhancement of MCAO success rate.