Primary human nasal epithelial cells: a source of poly (I:C) LMW-induced IL-6 production

Infection plays a significant role in the relapse of chronic rhinosinusitis (CRS), however, the role of primary human nasal epithelial cells (HNECs) in this process is largely unknown. Here, we determined the effect of Toll-like receptor (TLR) agonists and inflammatory cytokines on mucosal barrier i...

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Bibliographic Details
Published in:Scientific reports 2018-07, Vol.8 (1), p.11325-8, Article 11325
Main Authors: Ramezanpour, Mahnaz, Bolt, Harrison, Psaltis, Alkis James, Wormald, Peter-John, Vreugde, Sarah
Format: Article
Language:English
Subjects:
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Chronic infection
Cytokines
Enzyme-linked immunosorbent assay
Epithelial cells
Humanities and Social Sciences
Immune response
Inflammation
Interferon
Interleukin 6
Mucosal immunity
multidisciplinary
Poly (I:C)
Rhinosinusitis
Science
Science (multidisciplinary)
Small intestine
Toll-like receptors
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Summary:Infection plays a significant role in the relapse of chronic rhinosinusitis (CRS), however, the role of primary human nasal epithelial cells (HNECs) in this process is largely unknown. Here, we determined the effect of Toll-like receptor (TLR) agonists and inflammatory cytokines on mucosal barrier integrity and immune response of HNECs. TLR 1–9 agonists and inflammatory cytokines were applied to submerged and/or air-liquid interface (ALI) cultures of HNECs from CRS patients and controls for 24 hours. Interleukin-6 (IL-6) protein levels were determined by ELISA. Mucosal barrier integrity was measured via Transepithelial Electrical Resistance and passage of fluorescently-labelled dextrans. IL-1β and IFN- γ significantly increased IL-6 production in HNECs derived from CRS patients and controls, however, a dose-dependent effect was observed in CRS-derived HNECs only. Stimulation with Poly (I:C) LMW induced a 15 to 17 fold increase in IL-6 production by HNEC-ALI control cells (p 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-29765-0