Biochemical and Morphological Characterization of a Guanine Nucleotide Exchange Factor ARHGEF9 in Mouse Tissues

ARHGEF9, also known as Collybistin, a guanine nucleotide exchange factor for Rho family GTPases, is thought to play an essential role in the mammalian brain. In this study, we prepared a specific polyclonal antibody against ARHGEF9, anti-ARHGEF9, and carried out expression analyses with mouse tissue...

Full description

Saved in:
Bibliographic Details
Published in:ACTA HISTOCHEMICA ET CYTOCHEMICA 2018/06/26, Vol.51(3), pp.119-128
Main Authors: Ibaraki, Kyoko, Mizuno, Makoto, Aoki, Hitomi, Niwa, Ayumi, Iwamoto, Ikuko, Hara, Akira, Tabata, Hidenori, Ito, Hidenori, Nagata, Koh-ichi
Format: Article
Language:English
Subjects:
antibody
ARHGEF9
Cerebellum
Cerebral cortex
Dendritic spines
Developmental stages
Guanine
Guanine nucleotide exchange factor
guanine nucleotide exchange factor (GEF)
Hippocampus
Localization
neuron
Polyclonal antibodies
Regular
Western blotting
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ARHGEF9, also known as Collybistin, a guanine nucleotide exchange factor for Rho family GTPases, is thought to play an essential role in the mammalian brain. In this study, we prepared a specific polyclonal antibody against ARHGEF9, anti-ARHGEF9, and carried out expression analyses with mouse tissues especially brain. Western blotting analyses demonstrated tissue-dependent expression profiles of ARHGEF9 in the young adult mouse, and strongly suggested a role during brain development. Immunohistochemical analyses revealed developmental stage-dependent expression profiles of ARHGEF9 in cerebral cortex, hippocampus and cerebellum. ARHGEF9 exhibited partial localization at dendritic spines in cultured hippocampal neurons. From the obtained results, anti-ARHGEF9 was found to be a useful tool for biochemical and cell biological analyses of ARHGEF9.
ISSN:0044-5991
1347-5800
DOI:10.1267/ahc.18009