The Protective Effect of New Compound XH-103 on Radiation-Induced GI Syndrome

• Published in: Oxidative medicine and cellular longevity 2018-01, Vol.2018 (2018), p.1-9
• Main Authors: Li, Deguan, Wu, Jing, Hou, Qinlian, Cheng, Ying, Dong, Yinping, Tian, Hongqi
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Apoptosis; Apoptosis - drug effects; Apoptosis - radiation effects; Benzopyrans - chemical synthesis; Benzopyrans - chemistry; Benzopyrans - pharmacology; Bone marrow; Cancer therapies; Cell cycle; Deoxyribonucleic acid; DNA; DNA Damage; Drug dosages; Ethylenediaminetetraacetic acid; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Intestinal Mucosa - radiation effects; Intestine, Small - drug effects; Intestine, Small - metabolism; Intestine, Small - pathology; Intestine, Small - radiation effects; Ionizing radiation; Kinases; Laboratory animals; Male; Medical research; Mice; Mice, Inbred C57BL; Nuclear energy; Radiation Injuries, Experimental - drug therapy; Radiation Injuries, Experimental - metabolism; Radiation Injuries, Experimental - prevention & control; Radiation-Protective Agents - chemical synthesis; Radiation-Protective Agents - chemistry; Radiation-Protective Agents - pharmacology; Radiotherapy; Random Allocation; Science; Small intestine; Thiazolidines - chemical synthesis; Thiazolidines - chemistry; Thiazolidines - pharmacology; Tumor proteins; Tumor Suppressor Protein p53 - metabolism; Whole-Body Irradiation - adverse effects
• ISSN: 1942-0900; 1942-0994
• DOI: 10.1155/2018/3920147

Background. Radiation-induced intestinal injury is one of the side effects in patients receiving radiotherapy. The aim of the present study was to investigate the protective effect of XH-103 on radiation-induced small intestinal injury and to explore its mechanism. Methods. C57BL/6N mice were irradiated and treated with XH-103. Firstly, the survival rate of mice exposed to 9.0 Gy and 11.0 Gy total body irradiation (TBI) was examined. Subsequently, at 3.5 d after IR, the small intestinal morphological changes were examined by HE. The numbers of crypt cells, the villus height, the expression of Ki67 and Lgr5, and the apoptotic cells in the intestinal crypts were examined by immunohistochemistry. Furthermore, the expression of p53 and Bax was analyzed by WB. Results. Compared to the irradiation group, XH-103 improved the mice survival rate, protected the intestinal morphology of mice, decreased the apoptotic rate of intestinal crypt cells, maintained cell regeneration, and promoted crypt proliferation and differentiation. XH-103 also reduced the expression of p53 and Bax in the small intestine compared to the IR group. Conclusion. These data demonstrate that XH-103 can prevent radiation-induced intestinal injury, which is beneficial for the protection of radiation injuries.