Combination of Heparin Binding Peptide and Heparin Cell Surface Coatings for Mesenchymal Stem Cell Spheroid Assembly

Microtissues containing multiple cell types have been used in both in vitro models and in vivo tissue repair applications. However, to improve throughput, there is a need to develop a platform that supports self-assembly of a large number of 3D microtissues containing multiple cell types in a dynami...

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Bibliographic Details
Published in:Bioconjugate chemistry 2018-04, Vol.29 (4), p.878-884
Main Authors: Lei, Jennifer, Murphy, William L, Temenoff, Johnna S
Format: Article
Language:English
Subjects:
Anticoagulants
Binding
Binding sites
Cell Aggregation
Cell culture
Cell surface
Cells, Cultured
Cellular biology
Coatings
Heparin
Heparin - metabolism
Humans
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - metabolism
Mesenchyme
Peptides
Peptides - metabolism
Protective coatings
Self-assembly
Spheroids
Spheroids, Cellular - cytology
Spheroids, Cellular - metabolism
Stem cells
Suspension systems
Tissue engineering
Tissue Engineering - methods
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Summary:Microtissues containing multiple cell types have been used in both in vitro models and in vivo tissue repair applications. However, to improve throughput, there is a need to develop a platform that supports self-assembly of a large number of 3D microtissues containing multiple cell types in a dynamic suspension system. Thus, the objective of this study was to exploit the binding interaction between the negatively charged glycosaminoglycan, heparin, and a known heparin binding peptide to establish a method that promotes assembly of mesenchymal stem cell (MSC) spheroids into larger aggregates. We characterized heparin binding peptide (HEPpep) and heparin coatings on cell surfaces and determined the specificity of these coatings in promoting assembly of MSC spheroids in dynamic culture. Overall, combining spheroids with both coatings promoted up to 70 ± 11% of spheroids to assemble into multiaggregate structures, as compared to only 10 ± 4% assembly when cells having the heparin coating were cultured with cells coated with a scrambled peptide. These results suggest that this self-assembly method represents an exciting approach that may be applicable for a wide range of applications in which cell aggregation is desired.
ISSN:1043-1802
1520-4812
DOI:10.1021/acs.bioconjchem.7b00757