Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange

In this study, the three-dimensional spatial distributions of a number of metabolites involved in regulating symbiosis and biological nitrogen fixation (BNF) within soybean root nodules were revealed using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). While many...

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Bibliographic Details
Published in:The ISME Journal 2018-09, Vol.12 (9), p.2335-2338
Main Authors: Veličković, Dušan, Agtuca, Beverly J., Stopka, Sylwia A., Vertes, Akos, Koppenaal, David W., Paša-Tolić, Ljiljana, Stacey, Gary, Anderton, Christopher R.
Format: Article
Language:English
Subjects:
101/58
631/326
631/449
Adenosylmethionine
Bacteria
BASIC BIOLOGICAL SCIENCES
Biomedical and Life Sciences
Brief Communication
Complexity
Desorption
Ecology
Evolutionary Biology
Glycine max - metabolism
Glycine max - microbiology
Host plants
Ionization
Ions
Life Sciences
Mass spectrometry
Mass spectroscopy
Metabolism
Metabolites
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Nitrogen Fixation
Nitrogen-fixing bacteria
Nodules
Rhizobiaceae - metabolism
Root nodules
Root Nodules, Plant - metabolism
S-Adenosylmethionine
Skewed distributions
Soybeans
Spatial distribution
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Symbiosis
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Summary:In this study, the three-dimensional spatial distributions of a number of metabolites involved in regulating symbiosis and biological nitrogen fixation (BNF) within soybean root nodules were revealed using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). While many metabolites exhibited distinct spatial compartmentalization, some metabolites were asymmetrically distributed throughout the nodule (e.g., S-adenosylmethionine). These results establish a more complex metabolic view of plant–bacteria symbiosis (and BNF) within soybean nodules than previously hypothesized. Collectively these findings suggest that spatial perspectives in metabolic regulation should be considered to unravel the overall complexity of interacting organisms, like those relating to associations of nitrogen-fixing bacteria with host plants.
ISSN:1751-7362
1751-7370
DOI:10.1038/s41396-018-0188-8