Sexual Dimorphism in the Behavioral Responses and the Immunoendocrine Status in d-Galactose-Induced Aging

• Published in: The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 2018-08, Vol.73 (9), p.1147-1157
• Main Authors: Baeta-Corral, Raquel, Castro-Fuentes, Rafael, Giménez-Llort, Lydia
• Format: Article
• Language: English
• Subjects: Aging; Aging - metabolism; Aging - psychology; Aging, Premature - chemically induced; Aging, Premature - metabolism; Aging, Premature - psychology; Animals; D-Galactose; Dose-Response Relationship, Drug; Female; Fruits; Galactose; Galactose - metabolism; Galactose - pharmacology; Learning; Male; Maze Learning - physiology; Memory; Memory - drug effects; Memory - physiology; Mice; Milk; Motor Activity - drug effects; Motor Activity - physiology; Oxidative Stress; Reactive Oxygen Species - metabolism; Sex Factors; Sexual dimorphism; The Journal of Gerontology: Biological Sciences; Vegetables
• ISSN: 1079-5006; 1758-535X
• DOI: 10.1093/gerona/gly031

Abstract For almost 20 years, chronic systemic d-galactose, a monosaccharide abundantly present in milk products, fruits, and vegetables, has been used as a tool to achieve models of accelerated aging. Its neurotoxicity, induced by abnormal accumulation of reactive oxygen species and advanced glycation end products, has been widely reported. However, behavioral outcomes are still controversial and little is known about sex-dependent vulnerability. We performed a comprehensive behavioral and multifunctional screening of the chronic effects of low (50 mg/kg) and high (100 mg/kg) doses of d-galactose in 6-month-old male and female gold-standard C57BL/6 mice. Twelve classical tests with convergent validity analyzed sensorimotor, emotional and cognitive domains, indicating the existence of thresholds of response. Distinct vulnerability patterns were found in a selective sex- and dose-dependent manner. In males, d-galactose induced sensorimotor impairment and immunoendocrine senescence, but the low dose resulted in improved learning and memory. Oppositely, d-galactose-treated females exhibited a dose-dependent worse motor and spatial learning, but improved memory. Behavioral outcome items point at distinct neuronal substrates underlying the functional capacity of d-galactose-treated animals to meet task-dependent performance demands. They support that males and females can be regarded as two exceptional natural scenarios to study the functional interplay in the cross talk of homeostatic networks in aging.