Dietary chlorogenic acid improves growth performance of weaned pigs through maintaining antioxidant capacity and intestinal digestion and absorption function

• Published in: Journal of animal science 2018-04, Vol.96 (3), p.1108-1118
• Main Authors: Chen, Jiali, Li, Yan, Yu, Bing, Chen, Daiwen, Mao, Xiangbing, Zheng, Ping, Luo, Junqiu, He, Jun
• Format: Article
• Language: English
• Subjects: Absorption; Alkaline phosphatase; Animal Feed - analysis; Animals; Antioxidants; Antioxidants - metabolism; Biological activity; Catalase; Catalase - blood; Catalase - metabolism; Chlorogenic acid; Chlorogenic Acid - pharmacology; Diarrhea; Diarrhea - prevention & control; Diarrhea - veterinary; Diet; Diet - veterinary; Dietary Supplements; Digestibility; Digestion; Digestion - drug effects; Divalent metal transporter-1; Duodenum; Female; Gastrointestinal Tract - metabolism; Gene expression; Glucose; Glucose transport; Glucose transporter; Glutathione; Glutathione peroxidase; Glutathione Peroxidase - blood; Glutathione Peroxidase - metabolism; Hogs; Ileum; Incidence; Insulin-like growth factor I; Intestine; Jejunum; Male; mRNA; Na+/glucose cotransporter; Non Ruminant Nutrition; Nutrition; Peroxidase; Phenolic acids; Phenols; Protein transport; Proteins; Random Allocation; Serum albumin; Sodium; Superoxide dismutase; Superoxide Dismutase - blood; Superoxide Dismutase - metabolism; Swine - physiology; Swine Diseases - prevention & control; Urea; Weaning; Zinc
• ISSN: 0021-8812; 1525-3163
• DOI: 10.1093/jas/skx078

Abstract Chlorogenic acid (CGA) is a natural phenolic acid, which is an important component of biologically active dietary phenols isolated from various species. Two experiments were conducted to investigate the effects of CGA on growth performance, antioxidant capacity, nutrient digestibility, diarrhea incidence, intestinal digestion and absorption function, and the expression levels of intestinal digestion and absorption-related genes in weaned pigs. In Exp. 1, 200 weaned pigs were randomly allotted to four dietary treatments and fed with a basal diet or a basal diet supplemented with 250, 500, or 1,000 mg/kg CGA, respectively, in a 14-d trial. Pigs on the 1,000 mg/kg CGA-supplemented group had greater (P < 0.05) G:F compared with those on the control (CON) group. In Exp. 2, 24 weaned pigs were randomly allotted to two groups and fed with a basal diet (CON group) or a basal diet supplemented with 1,000 mg/kg CGA (the optimum does from Exp. 1; CGA group). After a 14-d trial, 8 pigs per treatment were randomly selected to collect serum and intestinal samples. Compared with the CON group, the ADG, G:F, as well as the apparent total tract digestibility of CP, crude fat, and ash were increased (P < 0.05), whereas the diarrhea incidence was decreased (P < 0.05) in the CGA group. Pigs on the CGA group had greater (P < 0.05) serum albumin and IGF-1, and lower (P < 0.05) serum urea nitrogen than pigs on the CON group. Furthermore, dietary CGA supplementation enhanced (P < 0.05) the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in the serum, the activity of maltase in the jejunum and ileum, as well as the activities of sucrase and alkaline phosphatase (AKP) in the jejunum. The mRNA levels of sodium glucose transport protein-1 (SGLT1) and zinc transporter-1 (ZNT1) in the duodenum and the mRNA levels of SGLT1, glucose transporter-2 (GLUT2), and divalent metal transporter-1 (DMT1) in the jejunum were upregulated (P < 0.05) in pigs fed the CGA diet. These results suggested that dietary CGA supplementation has the potentials to improve the growth performance and decrease the diarrhea incidence of the weaned pigs, possibly through improving the antioxidant capacity and enhancing the intestinal digestion and absorption function.