Molecular characterization of hepatitis A virus from children hospitalized at a tertiary care centre in northwest India

Background & objectives: Hepatitis A virus (HAV) infection is a major cause of childhood hepatitis, prevalent worldwide. HAV is classified into seven genotypes I-VII; genotypes III and I are the most common among humans. The present work was carried out to identify the genotypes prevalent in chi...

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Bibliographic Details
Published in:Indian journal of medical research (New Delhi, India : 1994) India : 1994), 2018-05, Vol.147 (5), p.507-512
Main Authors: Malhotra, Bharti, Kanwar, Anu, Reddy, P, Chauhan, Aradhana, Tiwari, Jitendra, Bhargava, Shipra, Verma, H
Format: Article
Language:English
Subjects:
Adolescent
Age
Child
Child, Preschool
Children
Confidence intervals
Diseases
Female
Genetic diversity
Genomes
Genotype
Genotype & phenotype
Health aspects
Hepatitis
Hepatitis A - genetics
Hepatitis A virus
Hepatitis A virus - genetics
Hepatitis A virus - isolation & purification
Hospitalization
Humans
India
Infant
Infant, Newborn
Laboratories
Male
Mortality
Original
Phylogenetics
Phylogeny
Physiological aspects
Polymerase chain reaction
RNA, Viral
Studies
Tertiary Care Centers
Virology
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Summary:Background & objectives: Hepatitis A virus (HAV) infection is a major cause of childhood hepatitis, prevalent worldwide. HAV is classified into seven genotypes I-VII; genotypes III and I are the most common among humans. The present work was carried out to identify the genotypes prevalent in children suspected to have acute viral hepatitis (AVH), hospitalized at a tertiary care centre in northwest India. Methods: A total of 1269 blood samples from children (0-15 yr of age) clinically suspected of viral hepatitis were screened for anti-HAV IgM. Acute phase serum was processed for RNA extraction and amplified by nested polymerase chain reaction (PCR) followed by sequencing of representative samples. Results: Among the 1269 samples tested, 642 (50.59%) were positive for anti-HAV IgM; among the positive samples, 171 patients having a history of less than seven days were tested by PCR, of whom 141 (82.45%) were found to be PCR positive. Nucleotide sequencing of a representative 44 samples showed high homology; all the samples were found to be of genotype IIIA. Interpretation & conclusions: Hepatitis A was prevalent during July to September and in predominantly children less than five years age. Only genotype IIIA was detected in all the samples.
ISSN:0971-5916
DOI:10.4103/ijmr.IJMR_1910_15