Quantitative trait loci analysis of brown blotch resistance in cowpea variety KN1

Cowpea is one of the most important crops in West Africa and is essential for the region’s food and nutrition security and economic development. Consequently, improving its agronomic performance and yield is a desirable goal. Brown blotch disease, caused by the fungal pathogen Colletotrichum capsici...

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Bibliographic Details
Published in:Molecular breeding 2018, Vol.38 (9), p.110-8, Article 110
Main Authors: Ohlson, Erik W., Thio, Gilles I., Sawadogo, Mahamadou, Sérémé, Paco, Timko, Michael P.
Format: Article
Language:English
Subjects:
Agronomy
Biomedical and Life Sciences
Biotechnology
Brown blotch
Chromosomes
Cowpeas
Cultivars
Disease control
Economic development
Food security
Gene mapping
Genetic resources
Genotype & phenotype
Inoculation
Life Sciences
Mapping
Markers
Molecular biology
Nutrition
Plant biology
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Polymerase chain reaction
Population genetics
Quantitative trait loci
Vigna unguiculata
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Summary:Cowpea is one of the most important crops in West Africa and is essential for the region’s food and nutrition security and economic development. Consequently, improving its agronomic performance and yield is a desirable goal. Brown blotch disease, caused by the fungal pathogen Colletotrichum capsici , is an important constraint of cowpea productivity, and at present, only limited genetic resources are available for breeding improved brown blotch-resistant varieties. The current study has characterized the genetic basis for brown blotch resistance conferred by the cowpea cultivar KN1 and identified a major dominant quantitative trait locus (QTL) for resistance on chromosome Vu02. A segregating F 2 population ( n  = 200), derived from a cross between KN1 and brown blotch-susceptible Tiligre (KVx775-33-2G), was developed and scored for disease severity following controlled inoculation. A subset of the population ( n  = 94) was genotyped with 99 newly developed allele-specific polymerase chain reaction (AS-PCR) markers, and multiple interval mapping was performed. One major and three minor QTL were identified. This is the first reported mapping of QTL conferring resistance to C. capsici in cowpea, and it is expected that the markers identified here will be a valuable resource for developing elite cowpea cultivars with resistance to brown blotch.
ISSN:1380-3743
1572-9788
DOI:10.1007/s11032-018-0867-1