Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions

Rapidly evolving cell-based therapies towards clinical trials demand alternative approaches for efficient expansion of adherent cell types such as human mesenchymal stem cells (hMSCs). Using microcarriers (100–300 µm) in a stirred tank bioreactor offers considerably enhanced surface to volume ratio...

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Bibliographic Details
Published in:Scientific reports 2018-08, Vol.8 (1), p.12481-10, Article 12481
Main Authors: Moloudi, Reza, Oh, Steve, Yang, Chun, Teo, Kim Leng, Lam, Alan Tin-Lun, Warkiani, Majid Ebrahimi, Naing, May Win
Format: Article
Language:English
Subjects:
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631/1647/277
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Bioreactors
Cell culture
Cell suspensions
Clinical trials
Flow rates
Humanities and Social Sciences
Mesenchyme
multidisciplinary
Purification
Reynolds number
Science
Science (multidisciplinary)
Shear stress
Stem cells
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Summary:Rapidly evolving cell-based therapies towards clinical trials demand alternative approaches for efficient expansion of adherent cell types such as human mesenchymal stem cells (hMSCs). Using microcarriers (100–300 µm) in a stirred tank bioreactor offers considerably enhanced surface to volume ratio of culture environment. However, downstream purification of the harvested cell product needs to be addressed carefully due to distinctive features and fragility of these cell products. This work demonstrates a novel alternative approach which utilizes inertial focusing to separate microcarriers (MCs) from the final cell suspension. First, we systematically investigated MC focusing dynamics inside scaled-up curved channels with trapezoidal and rectangular cross-sections. A trapezoidal spiral channel with ultra-low-slope (Tan(α) = 0.0375) was found to contribute to strong MC focusing (~300 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-31019-y