Comprehensive high-resolution multiple-reaction monitoring mass spectrometry for targeted eicosanoid assays

• Published in: Scientific data 2018-08, Vol.5 (1), p.180167-180167, Article 180167
• Main Authors: Sorgi, Carlos Artério, Peti, Ana Paula Ferranti, Petta, Tania, Meirelles, Alyne Fávero Galvão, Fontanari, Caroline, Moraes, Luiz Alberto Beraldo de, Faccioli, Lúcia Helena
• Format: Article
• Language: English
• Subjects: 631/92/608; 639/638/11/296; Data Descriptor; Eicosanoids; Fatty acids; Humanities and Social Sciences; Lipids; Mass spectroscopy; multidisciplinary; Science
• ISSN: 2052-4463; 2052-4463
• DOI: 10.1038/sdata.2018.167

Eicosanoids comprise a class of bioactive lipids derived from a unique group of essential fatty acids that mediate a variety of important physiological functions. Owing to the structural diversity of these lipids, their analysis in biological samples is often a major challenge. Advancements in mass spectrometric have been helpful for the characterization and quantification of these molecular lipid species in complex matrices. However, there are technical limitations to this approach, including low-abundant and/or poorly ionizable lipids. Using high-resolution multiple-reaction monitoring (MRM HR ), we were able to develop a targeted bioanalytical method for eicosanoid quantification. For this, we optimized the LC-MS/MS conditions and evaluated several parameters, including linearity, limits of quantification, matrix effects and recovery yields. For validation purposes, we looked at the method’s precision and accuracy. A library of high-resolution fragmentation spectra for eicosanoids was developed. Our comprehensive dataset meets benchmark standards for targeted analysis, having been derived using best-practice workflows and rigorous quality assessments. As such, our method has applications for determining complex eicosanoid profiles in the biomedical field. Design Type(s) replicate design • quality control testing design Measurement Type(s) Calibration • calibration substance role Technology Type(s) liquid chromatography-tandem mass spectrometry Factor Type(s) Matrix • Concentration • pH • technical replicate Machine-accessible metadata file describing the reported data (ISA-Tab format)