Oral human papillomavirus infection in England and associated risk factors: a case–control study

• Published in: BMJ open 2018-08, Vol.8 (8), p.e022497-e022497
• Main Authors: Hearnden, Vanessa, Murdoch, Craig, D’Apice, Katy, Duthie, Susan, Hayward, Nicholas J, Powers, Hilary Jane
• Format: Article
• Language: English
• Subjects: Alcohol; Cancer; Dentistry and Oral Medicine; DNA methylation; Head & neck cancer; Health risk assessment; Human papillomavirus; Immunization; Infections; Population; Questionnaires; Risk factors; Sample size; Sexual behavior; Smoking; Vitamin B; Women
• ISSN: 2044-6055; 2044-6055
• DOI: 10.1136/bmjopen-2018-022497

ObjectivesThis study was conducted to determine the prevalence of and associated risk factors for infection with oral high-risk human papillomavirus (HR-HPV) in adult participants within England, and to explore any association with oral mucosal buccal epithelial cell and whole blood folate concentration.DesignThis was an observational study to determine oral HR-HPV prevalence in the study population. A case–control study was performed to explore the association between infection and folate status.SettingThis study was conducted in Sheffield, UK, between April 2013 and August 2014.ParticipantsSeven hundred participants, aged 18–60 years, were recruited from university students (n=179), university and hospital staff (n=163), dental hospital patients (n=13), Sexual Health Sheffield patients (n=122) and the general public (n=223).InterventionsParticipants completed a lifestyle and sexual behaviour questionnaire, provided an oral rinse and gargle sample for the detection of oral HR-HPV and an oral mucosal buccal epithelial cell sample for the measurement of oral mucosal buccal epithelial cell folate. A blood sample was collected for measurement of whole blood folate concentration.Outcome measuresThe prevalence of oral HR-HPV infection in the study population was the primary outcome measure. Secondary outcome measures included associations between risk factors, folate status and infection.ResultsThe prevalence of oral HR-HPV infection in this cohort was 2.2% (15/680) with 0.7% (5/680) positive for HPV16 or HPV18. Twenty samples were excluded due to insufficient material for HPV detection. Participants with oral HR-HPV infection were more likely to be a former smoker, and have a greater number of sexual and oral sexual partners. Folate status was not linked to likelihood of HPV infection.ConclusionsThe prevalence of oral infection with HR-HPV in adult men and women in Sheffield in the North of England was low. Smoking and sexual behaviour were associated with HR-HPV positivity.Trial registration numberID14106.