Oocyte-specific gene Oog1 suppresses the expression of spermatogenesis-specific genes in oocytes

Oog1, an oocyte-specific gene that encodes a protein of 425 amino acids, is present in five copies on mouse chromosomes 4 and 12. In mouse oocytes, Oog1 mRNA expression begins at embryonic day 15.5 and almost disappears by the late two-cell stage. Meanwhile, OOG1 protein is detectable in oocytes in...

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Bibliographic Details
Published in:Journal of Reproduction and Development 2018, Vol.64(4), pp.297-301
Main Authors: HONDA, Shinnosuke, MIKI, Yuka, MIYAMOTO, Yuya, KAWAHARA, Yu, TSUKAMOTO, Satoshi, IMAI, Hiroshi, MINAMI, Naojiro
Format: Article
Language:English
Subjects:
Cysts
Double-stranded RNA
Flagella
Gene expression
Genes
Germ cells
Microtubules
Mouse
Oocytes
Oog1
Oogenesis
Original
Ovaries
Polymerase chain reaction
Protein transport
Proteins
RNA-mediated interference
RNAi
siRNA
Sperm
Spermatogenesis
Transgenic mice
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Summary:Oog1, an oocyte-specific gene that encodes a protein of 425 amino acids, is present in five copies on mouse chromosomes 4 and 12. In mouse oocytes, Oog1 mRNA expression begins at embryonic day 15.5 and almost disappears by the late two-cell stage. Meanwhile, OOG1 protein is detectable in oocytes in ovarian cysts and disappears by the four-cell stage; the protein is transported to the nucleus in late one-cell to early two-cell stage embryos. In this study, we examined the role of Oog1 during oogenesis in mice. Oog1 RNAi-transgenic mice were generated by expressing double-stranded hairpin Oog1 RNA, which is processed into siRNAs targeting Oog1 mRNA. Quantitative RT-PCR revealed that the amount of Oog1 mRNA was dramatically reduced in oocytes obtained from Oog1-knockdown mice, whereas the abundance of spermatogenesis-associated transcripts (Klhl10, Tekt2, Tdrd6, and Tnp2) was increased in Oog1 knockdown ovaries. Tdrd6 is involved in the formation of the chromatoid body, Tnp2 contributes to the formation of sperm heads, Tekt2 is required for the formation of ciliary and flagellar microtubules, and Klhl10 plays a key role in the elongated sperm differentiation. These results indicate that Oog1 down-regulates the expression of spermatogenesis-associated genes in female germ cells, allowing them to develop normally into oocytes.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.2018-024