Photo-Induced Electron Transfer Real-Time PCR for Detection of Plasmodium falciparum plasmepsin 2 Gene Copy Number

Piperaquine is an important partner drug used in artemisinin-based combination therapies (ACTs). An increase in the and gene copy numbers has been associated with decreased susceptibility of to piperaquine in Cambodia. Here, we developed a photo-induced electron transfer real-time PCR (PET-PCR) assa...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2018-08, Vol.62 (8)
Main Authors: Souza, Samaly Santos, L'Episcopia, Mariangela, Severini, Carlo, Udhayakumar, Venkatachalam, Lucchi, Naomi W
Format: Article
Language:English
Subjects:
Antimalarials
Aspartic Acid Endopeptidases
Experimental Therapeutics
Gene Dosage
Plasmodium falciparum
Protozoan Proteins
Quinolines
Real-Time Polymerase Chain Reaction
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Summary:Piperaquine is an important partner drug used in artemisinin-based combination therapies (ACTs). An increase in the and gene copy numbers has been associated with decreased susceptibility of to piperaquine in Cambodia. Here, we developed a photo-induced electron transfer real-time PCR (PET-PCR) assay to quantify the copy number of the gene ( ) that can be used in countries where is endemic to enhance molecular surveillance.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.00317-18