Use of sucrose to diminish pore formation in freeze-dried heart valves

Freeze-dried storage of decellularized heart valves provides easy storage and transport for clinical use. Freeze-drying without protectants, however, results in a disrupted histoarchitecture after rehydration. In this study, heart valves were incubated in solutions of various sucrose concentrations...

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Bibliographic Details
Published in:Scientific reports 2018-08, Vol.8 (1), p.12982-12, Article 12982
Main Authors: Vásquez-Rivera, Andrés, Oldenhof, Harriëtte, Dipresa, Daniele, Goecke, Tobias, Kouvaka, Artemis, Will, Fabian, Haverich, Axel, Korossis, Sotirios, Hilfiker, Andres, Wolkers, Willem F.
Format: Article
Language:English
Subjects:
13/107
14/63
14/69
631/61/490
692/4019/592/2725
Biomechanics
Calorimetry
Collagen
Differential scanning calorimetry
Elastin
Fourier transforms
Freeze drying
Heart
Humanities and Social Sciences
Infrared spectroscopy
multidisciplinary
Pores
Porosity
Rehydration
Science
Science (multidisciplinary)
Sucrose
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Summary:Freeze-dried storage of decellularized heart valves provides easy storage and transport for clinical use. Freeze-drying without protectants, however, results in a disrupted histoarchitecture after rehydration. In this study, heart valves were incubated in solutions of various sucrose concentrations and subsequently freeze-dried. Porosity of rehydrated valves was determined from histological images. In the absence of sucrose, freeze-dried valves were shown to have pores after rehydration in the cusp, artery and muscle sections. Use of sucrose reduced pore formation in a dose-dependent manner, and pretreatment of the valves in a 40% (w/v) sucrose solution prior to freeze-drying was found to be sufficient to completely diminish pore formation. The presence of pores in freeze-dried valves was found to coincide with altered biomechanical characteristics, whereas biomechanical parameters of valves freeze-dried with enough sucrose were not significantly different from those of valves not exposed to freeze-drying. Multiphoton imaging, Fourier transform infrared spectroscopy, and differential scanning calorimetry studies revealed that matrix proteins (i.e. collagen and elastin) were not affected by freeze-drying.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-31388-4