Genome-Wide Quantification of the Effect of Gene Overexpression on Escherichia coli Growth

Recombinant protein production plays an essential role in both biological studies and pharmaceutical production. is one of the most favorable hosts for this purpose. Although a number of strategies for optimizing protein production have been developed, the effect of gene overexpression on host cell...

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Bibliographic Details
Published in:Genes 2018-08, Vol.9 (8), p.414
Main Authors: Chen, Hao, Venkat, Sumana, Wilson, Jessica, McGuire, Paige, Chang, Abigail L, Gan, Qinglei, Fan, Chenguang
Format: Article
Language:English
Subjects:
Amino acids
Biochemistry
Biomass
Cell growth
E coli
Escherichia coli
Genes
Genomes
Metabolism
Molecular biology
Proteins
RNA polymerase
Supplements
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Summary:Recombinant protein production plays an essential role in both biological studies and pharmaceutical production. is one of the most favorable hosts for this purpose. Although a number of strategies for optimizing protein production have been developed, the effect of gene overexpression on host cell growth has been much less studied. Here, we performed high-throughput tests on the a complete set of K-12 ORF archive (ASKA) collection to quantify the effects of overexpressing individual genes on its growth. The results indicated that overexpressing membrane-associated proteins or proteins with high abundances of branched-chain amino acids tended to impair cell growth, the latter of which could be remedied by amino acid supplementation. Through this study, we expect to provide an index for a fast pre-study estimate of host cell growth in order to choose proper rescuing approaches when working with different proteins.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes9080414