Lysozyme and DNase I loaded poly (D, L lactide-co-caprolactone) nanocapsules as an oral delivery system

Clinical applications of oral protein therapy for the treatment of various chronic diseases are limited due to the harsh conditions encounter the proteins during their journey in the Gastrointestinal Tract. Although nanotechnology forms a platform for the development of oral protein formulations, ob...

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Bibliographic Details
Published in:Scientific reports 2018-09, Vol.8 (1), p.13158-10, Article 13158
Main Authors: Abu Abed, Omar S., Chaw, Cheng, Williams, Lee, Elkordy, Amal A.
Format: Article
Language:English
Subjects:
631/154/152
631/154/51/2314
Biological activity
Chronic illnesses
Deoxyribonuclease
Encapsulation
Gastrointestinal tract
Humanities and Social Sciences
Intestine
Lysozyme
Monomers
multidisciplinary
Nanotechnology
Proteins
Science
Science (multidisciplinary)
Therapeutic applications
Trehalose
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Summary:Clinical applications of oral protein therapy for the treatment of various chronic diseases are limited due to the harsh conditions encounter the proteins during their journey in the Gastrointestinal Tract. Although nanotechnology forms a platform for the development of oral protein formulations, obtaining physiochemically stable formulations able to deliver active proteins is still challenging because of harsh preparation conditions. This study proposes the use of poly (D, L-lactic-co-caprolactone)-based polymeric nanocapsules at different monomers’ ratios for protein loading and oral delivery. All formulations had a spherical shape and nano-scale size, and lysozyme encapsulation efficiency reached 80% and significantly affected by monomers’ ratio. Trehalose and physical state of lysozyme had a significant effect on its biological activity (P 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-31303-x