Bacterial Endospores as Phage Genome Carriers and Protective Shells

• Published in: Applied and environmental microbiology 2018-09, Vol.84 (18)
• Main Authors: Gabiatti, Naiana, Yu, Pingfeng, Mathieu, Jacques, Lu, Grant W, Wang, Xifan, Zhang, Hangjun, Soares, Hugo M, Alvarez, Pedro J J
• Format: Article
• Language: English
• Subjects: Alanine; Asparagine; Bacillus cereus - genetics; Bacillus cereus - growth & development; Bacillus cereus - virology; Bacillus Phages - chemistry; Bacillus Phages - genetics; Bacillus Phages - growth & development; Bacillus subtilis - genetics; Bacillus subtilis - growth & development; Bacillus subtilis - virology; Bacteria; Entrapment; Environmental conditions; Environmental stress; Fructose; Genome, Viral; Genomes; Germination; Glucose; High temperature; Hot Temperature; Hydrogen-Ion Concentration; L-Alanine; Low concentrations; Methods; Microorganisms; Multidrug resistant organisms; Multiplicity of infection; Opportunist infection; pH effects; Phages; Shells; Spores, Bacterial - chemistry; Spores, Bacterial - genetics; Spores, Bacterial - growth & development; Spores, Bacterial - virology
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.01186-18

Bacterial endospores can serve as phage genome protection shells against various environmental stresses to enhance microbial control applications. The genomes of polyvalent lytic phages PBSC1 and PBSC2, which infect both subsp. and NRS 248, were incorporated into endospores (without integration into the host chromosome). When PBSC1 and PBSC2 were released from germinating endospores, they significantly inhibited the growth of the targeted opportunistic pathogen Optimal endospore entrapment was achieved when phages were introduced to the fast-sporulating prespores at a multiplicity of infection of 1. Longer endospore maturation (48 h versus 24 h) increased both spore yield and efficiency of entrapment. Compared with free phages, spore-protected phage genomes showed significantly higher resistance toward high temperatures (60 to 80°C), extreme pH (pH 2 or pH 12), and copper ions (0.1 to 10 mg/liter). Endospore germination is inducible by low concentrations of l-alanine or by a germinant mixture (l-asparagine, d-glucose, d-fructose, and K ) to trigger the expression, assembly, and consequent release of phage particles within 60 to 90 min. Overall, the superior resiliency of polyvalent phages protected by endospores might enable nonrefrigerated phage storage and enhance phage applications after exposure to adverse environmental conditions. Bacteriophages are being considered for the control of multidrug-resistant and other problematic bacteria in environmental systems. However, the efficacy of phage-based microbial control is limited by infectivity loss during phage delivery and/or storage. Here, we exploit the pseudolysogenic state of phages, which involves incorporation of their genome into bacterial endospores (without integration into the host chromosome), to enhance survival in unfavorable environments. We isolated polyvalent (broad-host-range) phages that efficiently infect both benign and opportunistically pathogenic strains and encapsulated the phage genomes in endospores to significantly improve resistance to various environmental stressors. Encapsulation by spores also significantly enhanced phage genome viability during storage. We also show that endospore germination can be induced on demand with nutrient germinants that trigger the release of active phages. Overall, we demonstrate that encapsulation of polyvalent phage genomes into benign endospores holds great promise for broadening the scope and efficacy of phage biocontrol.