Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue

• Published in: Experimental and therapeutic medicine 2018-09, Vol.16 (3), p.1647-1654
• Main Authors: Karaarslan, Numan, Batmaz, Ahmet Guray, Yilmaz, Ibrahim, Ozbek, Hanefi, Caliskan, Tezcan, Yasar Sirin, Duygu, Kaplan, Necati, Oznam, Kadir, Ates, Ozkan
• Format: Article
• Language: English
• Subjects: Acids; Antigens; Cell culture; Cell growth; Cytotoxicity; Drug dosages; Experiments; Joint surgery; Laboratories; Medical research; Microscopy; Morphology; Proteins; Stem cells; Studies; Surgery; Values
• ISSN: 1792-0981; 1792-1015
• DOI: 10.3892/etm.2018.6351

Non-steroidal anti-inflammatory drugs (NSAIDs) that are applied through oral, injectable or topical routes have been widely used in painful and inflammatory musculoskeletal diseases. The current study aimed to determine whether naproxen, an aryl acetic acid derivative with analgesic and anti-inflammatory effects, has a toxic effect on human chondrocytes. Samples containing monolayer primary chondrocyte cultures were prepared following resection from osteochondral tissues obtained from patients with gonarthrosis. Cell viability, toxicity and proliferation and levels of stage-specific embryonic antigen-1, a precursor to human prechondrocytes, were evaluated spectrophotometrically. The results from the untreated control group were compared with those of the study groups, where naproxen was administered in varying doses (1-1,000 µM). Surface morphologies of the cells were compared using inverted light and environmental scanning electron microscopy. Treatment groups were compared by analysis of variance with Tukey's honest difference post hoc test. P