A novel prognostic marker and immunogenic membrane antigen: prohibitin (PHB) in pancreatic cancer

Previous study, using immunoblotting with IgG and membrane proteins, identified prohibitin (PHB) as a potential immunogenic membrane antigen. Now, investigate PHB expression and biological functions in pancreatic cancer. PHB expression was analysed in PDAC cell lines, normal pancreas tissues, cancer...

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Bibliographic Details
Published in:Clinical and translational gastroenterology 2018-09, Vol.9 (9), p.178-12
Main Authors: Wang, Weibin, Xu, Lai, Yang, Yu, Dong, LiangBo, Zhao, BangBo, Lu, Jun, Zhang, Taiping, Zhao, Yupei
Format: Article
Language:English
Subjects:
Aged
Antigens
Apoptosis
Biomarkers, Tumor - blood
Biomarkers, Tumor - metabolism
Blotting, Western
Cell growth
Cell Line, Tumor
Cell Movement
Cell Proliferation
Female
Flow Cytometry
Humans
Male
Neoplasm Invasiveness
Pancreas - metabolism
Pancreatic cancer
Pancreatic Neoplasms - metabolism
Pancreatic Neoplasms - pathology
Prognosis
Real-Time Polymerase Chain Reaction
Repressor Proteins - blood
Repressor Proteins - metabolism
RNA, Small Interfering
Survival Analysis
Transfection
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Summary:Previous study, using immunoblotting with IgG and membrane proteins, identified prohibitin (PHB) as a potential immunogenic membrane antigen. Now, investigate PHB expression and biological functions in pancreatic cancer. PHB expression was analysed in PDAC cell lines, normal pancreas tissues, cancer tissues, PDAC patient sera and healthy volunteer sera using QRT-PCR, Western blotting, IHC, and ELISA, and a survival analysis and a COX regression analysis were performed. Low and high PHB expression levels were accomplished using RNA interference technology and gene transfer techniques. Cell proliferation, migration, and invasion, apoptosis-related proteins were assessed 48 h after transfection. PHB was generally expressed in the 8 tested PDAC cell lines. PHB was significantly increased in PDAC tissues and negatively correlated with overall survival (p 
ISSN:2155-384X
2155-384X
DOI:10.1038/s41424-018-0044-1