LncRNA TUG1 promotes cells proliferation and inhibits cells apoptosis through regulating AURKA in epithelial ovarian cancer cells

• Published in: Medicine (Baltimore) 2018-09, Vol.97 (36), p.e12131-e12131
• Main Authors: Li, Tonghuai, Chen, Yan, Zhang, Jingjing, Liu, Shaoxiao
• Format: Article
• Language: English
• Subjects: Apoptosis - physiology; Aurora Kinase A - administration & dosage; Aurora Kinase A - metabolism; Carcinoma, Ovarian Epithelial; Cell Line, Tumor; Cell Proliferation - physiology; Claudin-3 - metabolism; Humans; Neoplasms, Glandular and Epithelial - metabolism; Observational Study; Ovarian Neoplasms - metabolism; Plasminogen Activator Inhibitor 1 - metabolism; Proto-Oncogene Protein c-ets-1 - metabolism; RNA, Long Noncoding - administration & dosage; RNA, Long Noncoding - antagonists & inhibitors; RNA, Long Noncoding - metabolism
• ISSN: 0025-7974; 1536-5964
• DOI: 10.1097/MD.0000000000012131

This study aimed to assess the effect of long noncoding RNAs (lncRNAs) taurine-upregulated gene 1 (TUG1) on cells proliferation and apoptosis as well as its targeting genes in epithelial ovarian cancer (EOC) cells.Blank mimic, lncRNA TUG1 mimic, blank inhibitor, and lncRNA TUG1 inhibitor plasmids were transfected into SK-OV-3 (SKOV3) cells. Rescue experiment was performed by the transfection of lncRNA TUG1 inhibitor and Aurora kinase A (AURKA) mimic plasmids into SKOV3 cells. Cell counting kit-8 (CKK-8), annexin V-FITC (AV)-propidium iodide (PI) (AV-PI), quantitative polymerase chain reaction (qPCR), and western blot assays were performed to detect cells proliferation, apoptosis, RNA expression, and protein expression respectively.Cells proliferation was increased in lncRNA TUG1 mimic group and decreased in lncRNA TUG1 inhibitor group than normal control (NC) groups. Cells apoptosis rate was repressed after treatment with lncRNA TUG1 mimic and promoted after treatment with lncRNA TUG1 inhibitor. AURKA expression but not CLDN3, SERPINE1, or ETS1 expression was adversely regulated by lncRNA TUG1 mimic and inhibitor. After transferring lncRNA TUG1 (-) and AURKA (+) plasmids, cells proliferation was increased, while cells apoptosis rate was decreased in AURKA mimic (+)/lncRNA TUG1 inhibitor (-) group than NC (+)/lncRNA TUG1 (-) group, which suggested lncRNA TUG1 regulated cells proliferation and cells apoptosis through targeting AURKA.LncRNA TUG1 promotes cells proliferation and inhibits cells apoptosis through regulating AURKA in EOC cells.