Evaluation of oxidative stress in caprine bronchoalveolar lavage fluid of pneumonic and normal lungs

Research in the area of oxidative stress in pneumonic pathology still requires attention in small ruminants especially with the use of bronchoalveolar lavage (BAL) which may be a more sensitive indicator of respiratory diseases than blood. This investigation evaluates the role of oxidative stressin...

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Bibliographic Details
Published in:International journal of veterinary science and medicine 2017-12, Vol.5 (2), p.143-147
Main Authors: Jarikre, T.A., Ohore, G.O., Oyagbemi, A.A., Emikpe, B.O.
Format: Article
Language:English
Subjects:
Anti-oxidants
Glutathione transferase
Malondialdehyde
Myeloperoxidase
Original
Pneumonia
Pro-oxidants
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Summary:Research in the area of oxidative stress in pneumonic pathology still requires attention in small ruminants especially with the use of bronchoalveolar lavage (BAL) which may be a more sensitive indicator of respiratory diseases than blood. This investigation evaluates the role of oxidative stressin the pathogenesis of caprine pneumonia using BAL fluid (BALf) from healthy and pneumonic goats. A BALf from 192 goats (whose pneumonic histopathology had been characterized using standard techniques) was biochemically assayed for anti-oxidants and pro-oxidants. Malondialdehyde (MDA), hydrogen peroxide generation (H2O2), myeloperoxidase (MPO) and reduced glutathione (GSH) contents were measured to assess free radical activity in the BALf. Superoxide dismutase (SOD), Glutathione transferase (GST) and Glutathione peroxidase (GPx) activity were also determined colourimetrically. There were significant increases in the BALf supernatant of MDA, H2O2 and MPO with decreases in GSH level and SOD activity in the pneumonic goats (P
ISSN:2314-4599
2314-4599
DOI:10.1016/j.ijvsm.2017.09.001