Chronic exercise impairs nitric oxide pathway in rabbit carotid and femoral arteries

• Published in: The Journal of physiology 2018-09, Vol.596 (18), p.4361-4374
• Main Authors: Marchio, Patricia, Guerra‐Ojeda, Solanye, Vila, José M., Aldasoro, Martín, Valles, Soraya L., Soler, Carlos, Mauricio, Maria D.
• Format: Article
• Language: English
• Subjects: Acetylcysteine; calcium activated potassium channels; Calcium conductance; Carotid arteries; Carotid artery; Charybdotoxin; chronic exercise; endothelial dysfunction; Exercise; Femoral artery; Femur; Isometric; Nitric oxide; Nitric-oxide synthase; Ouabain; Oxidative stress; Physical training; Potassium channels (calcium-gated); Potassium chloride; Potassium conductance; Research Paper; Superoxide dismutase; Vasodilation; Western blotting
• ISSN: 0022-3751; 1469-7793
• DOI: 10.1113/JP275611

Key points Some of the beneficial effects of exercise in preventing vascular related diseases are mediated by the enhancement of endothelial function where the role of nitric oxide (NO) is well documented, although the relevance of calcium activated potassium channels is not fully understood. The impact of oxidative stress induced by training on endothelial function remains to be clarified. By evaluating different endothelial vasodilator pathways on two vascular beds in a rabbit model of chronic exercise, we found a decreased NO bioavailability and endothelial nitric oxide synthase expression in both carotid and femoral arteries. Physical training induced carotid endothelial dysfunction as a result of an increase in oxidative stress and a reduction in superoxide dismutase expression. In the femoral artery, the lower production of NO was counteracted by an increased participation of large conductance calcium activated potassium channels, preventing endothelial dysfunction. The present study aimed to evaluate the effects of chronic exercise on vasodilator response in two different arteries. Rings of carotid and femoral arteries from control and trained rabbits were suspended in organ baths for isometric recording of tension. Endothelial nitric oxide synthase (eNOS), Cu/Zn and Mn‐superoxide dismutase (SOD), and large conductance calcium activated potassium (BKCa) channel protein expression were measured by western blotting. In the carotid artery, training reduced the relaxation to ACh (10–9 to 3 × 10–6 m) that was reversed by N‐acetylcysteine (10–3 m). l‐NAME (10–4 m) reduced the relaxation to ACh in both groups, although the effect was lower in the trained group (in mean ± SEM, 39 ± 2% vs. 28 ± 3%). Physical training did not modify the relaxation to ACh in femoral arteries, although the response to l‐NAME was lower in the trained group (in mean ± SEM, 41 ± 5% vs. 17 ± 2%). Charybdotoxin (10–7 m) plus apamin (10–6 m) further reduced the maximal relaxation to ACh only in the trained group. The remaining relaxation in both carotid and femoral arteries was abolished by KCl (2 × 10–2 m) and BaCl2 (3 × 10–6 m) plus ouabain (10–4 m) in both groups. Physical training decreased eNOS expression in both carotid and femoral arteries and Cu/Zn and Mn‐SOD expression only in the carotid artery. BKCa channels were overexpressed in the trained group in the femoral artery. In conclusion, chronic exercise induces endothelial dysfunction in the carotid artery as a result of oxi