MiR-199a-5p regulates sirtuin1 and PI3K in the rat hippocampus with intrauterine growth restriction

In humans, malnutrition during pregnancy results in intrauterine growth restriction (IUGR) and an increased risk of neurological morbidities; altered miRNA characteristics have been suggested to contribute to IUGR neurological pathogenesis. A miRNA microarray was used to identify differentially expr...

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Bibliographic Details
Published in:Scientific reports 2018-09, Vol.8 (1), p.13813-10, Article 13813
Main Authors: Chen, Juncao, Gong, Xiaoyun, Huang, Li, Chen, Pingyang, Wang, Tao, Zhou, Wei, Luo, Kaiju, Wang, Jing
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
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Animals
Bioinformatics
Caspase
Caspase 3 - genetics
Caspase 3 - metabolism
Caspase-3
Female
Fetal Growth Retardation - genetics
Fetal Growth Retardation - metabolism
Fetal Growth Retardation - pathology
Health risk assessment
Hippocampus
Hippocampus - metabolism
Hippocampus - pathology
Humanities and Social Sciences
Malnutrition
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
multidisciplinary
Neurons - metabolism
Neurons - pathology
Pathogenesis
Pheochromocytoma cells
Phosphatidylinositol 3-Kinases - genetics
Phosphatidylinositol 3-Kinases - metabolism
Pregnancy
Rats
Rats, Sprague-Dawley
Science
Science (multidisciplinary)
SIRT1 protein
Sirtuin 1 - genetics
Sirtuin 1 - metabolism
Tumor necrosis factor-α
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Summary:In humans, malnutrition during pregnancy results in intrauterine growth restriction (IUGR) and an increased risk of neurological morbidities; altered miRNA characteristics have been suggested to contribute to IUGR neurological pathogenesis. A miRNA microarray was used to identify differentially expressed miRNA molecules in the hippocampi of rats with IUGR. Five of the molecules in question were selectively validated using real-time PCR in rats with IUGR. We then investigated the role of miR-199a-5p in hippocampal pathology. Bioinformatics analysis results suggested that TNF-α, caspase-3 and SIRT1 were potential targets of miR-199a-5p. Changes in PI3K, SIRT1 and caspase-3 protein expressions levels in the hippocampus were confirmed by Western blot analysis (all P  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-32189-5