Nicotine and sleep deprivation: impact on pain sensitivity and immune modulation in rats

Repeated nicotine administration has been associated with increased paradoxical sleep in rats and antinociceptive properties, whereas paradoxical sleep deprivation (PSD) elicits pronociceptive and inflammatory responses. Thus, we aimed to evaluate the effect of repeated nicotine administration and i...

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Bibliographic Details
Published in:Scientific reports 2018-09, Vol.8 (1), p.13837-9, Article 13837
Main Authors: Hirotsu, Camila, Pedroni, Matheus Negrao, Berro, Laís Fernanda, Tufik, Sergio, Andersen, Monica Levy
Format: Article
Language:English
Subjects:
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692/617/375/1692
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Humanities and Social Sciences
Immunomodulation
Inflammation
Interleukin 4
Interleukin 6
Latency
Mecamylamine
multidisciplinary
Nicotine
Pain
Pain perception
REM sleep
Rodents
Science
Science (multidisciplinary)
Sleep deprivation
Tumor necrosis factor-α
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Summary:Repeated nicotine administration has been associated with increased paradoxical sleep in rats and antinociceptive properties, whereas paradoxical sleep deprivation (PSD) elicits pronociceptive and inflammatory responses. Thus, we aimed to evaluate the effect of repeated nicotine administration and its withdrawal combined with PSD on pain sensitivity and inflammatory markers. Sixty adult male Wistar rats were subjected to repeated injections of saline (SAL) or nicotine (NIC) for 12 days or 7 days of nicotine followed by acute mecamylamine administration on day 8 to precipitate nicotine abstinence (ABST). On day 9, the animals were submitted to PSD for 72 h or remained in control condition (CTRL); on day 12, thermal pain threshold was assessed by the hot plate test. PSD significantly decreased the latency to paw withdrawal in all groups compared to their respective controls. ABST-PSD animals presented higher levels of interleukin (IL)-6 compared to all groups, except ABST-CTRL. After adjustment for weight loss, IL-6, IL-4 and tumor necrosis factor alpha, ABST-PSD was associated with the lowest pain threshold. Nicotine and IL-4 levels were predictors of higher pain threshold. Hyperalgesia induced by PSD prevailed over the antinociceptive action of nicotine, while the association between PSD and ABST synergistically increased IL-6 concentrations and decreased pain threshold.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-32276-7