Whole Genome Incorporation and Epigenetic Stability in a Newly Synthetic Allopolyploid of Gynogenetic Gibel Carp

Allopolyploidization plays an important role in speciation, and some natural or synthetic allopolyploid fishes have been extensively applied to aquaculture. Although genetic and epigenetic inheritance and variation associated with plant allopolyploids have been well documented, the relative research...

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Bibliographic Details
Published in:Genome biology and evolution 2018-09, Vol.10 (9), p.2394-2407
Main Authors: Shao, Guang-Ming, Li, Xi-Yin, Wang, Yang, Wang, Zhong-Wei, Li, Zhi, Zhang, Xiao-Juan, Zhou, Li, Gui, Jian-Fang
Format: Article
Language:English
Subjects:
Amplified Fragment Length Polymorphism Analysis
Animals
Base Sequence
Carps - genetics
DNA Methylation
DNA, Mitochondrial - genetics
DNA, Ribosomal - genetics
Epigenesis, Genetic
Female
In Situ Hybridization, Fluorescence
Male
Polymorphism, Restriction Fragment Length
Polyploidy
Spermatozoa - metabolism
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Summary:Allopolyploidization plays an important role in speciation, and some natural or synthetic allopolyploid fishes have been extensively applied to aquaculture. Although genetic and epigenetic inheritance and variation associated with plant allopolyploids have been well documented, the relative research in allopolyploid animals is scarce. In this study, the genome constitution and DNA methylation inheritance in a newly synthetic allopolyploid of gynogenetic gibel carp were analyzed. The incorporation of a whole genome of paternal common carp sperm in the allopolyploid was confirmed by genomic in situ hybridization, chromosome localization of 45S rDNAs, and sequence comparison. Pooled sample-based methylation sensitive amplified polymorphism (MSAP) revealed that an overwhelming majority (98.82%) of cytosine methylation patterns in the allopolyploid were inherited from its parents of hexaploid gibel carp clone D and common carp. Compared to its parents, 11 DNA fragments in the allopolyploid were proved to be caused by interindividual variation, recombination, deletion, and mutation through individual sample-based MSAP and sequencing. Contrast to the rapid and remarkable epigenetic changes in most of analyzed neopolyploids, no cytosine methylation variation was detected in the gynogenetic allopolyploid. Therefore, the newly synthetic allopolyploid of gynogenetic gibel carp combined genomes from its parents and maintained genetic and epigenetic stability after its formation and subsequently seven successive gynogenetic generations. Our current results provide a paradigm for recurrent polyploidy consequences in the gynogenetic allopolyploid animals.
ISSN:1759-6653
1759-6653
DOI:10.1093/gbe/evy165