CRISPR-Cas immunity, DNA repair and genome stability

Co-opting of CRISPR-Cas 'Interference' reactions for editing the genomes of eukaryotic and prokaryotic cells has highlighted crucial support roles for DNA repair systems that strive to maintain genome stability. As front-runners in genome editing that targets DNA, the class 2 CRISPR-Cas en...

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Bibliographic Details
Published in:Bioscience reports 2018-10, Vol.38 (5)
Main Authors: Cubbon, Andrew, Ivancic-Bace, Ivana, Bolt, Edward L
Format: Article
Language:English
Subjects:
CRISPR-Associated Protein 9 - genetics
CRISPR-Cas Systems - genetics
DNA Repair - genetics
Gene Editing
Genome, Human - genetics
Genomic Instability - genetics
Humans
Review
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Summary:Co-opting of CRISPR-Cas 'Interference' reactions for editing the genomes of eukaryotic and prokaryotic cells has highlighted crucial support roles for DNA repair systems that strive to maintain genome stability. As front-runners in genome editing that targets DNA, the class 2 CRISPR-Cas enzymes Cas9 and Cas12a rely on repair of DNA double-strand breaks (DDSBs) by host DNA repair enzymes, using mechanisms that vary in how well they are understood. Data are emerging about the identities of DNA repair enzymes that support genome editing in human cells. At the same time, it is becoming apparent that CRISPR-Cas systems functioning in their native environment, bacteria or archaea, also need DNA repair enzymes. In this short review, we survey how DNA repair and CRISPR-Cas systems are intertwined. We consider how understanding DNA repair and CRISPR-Cas interference reactions in nature might help improve the efficacy of genome editing procedures that utilise homologous or analogous systems in human and other cells.
ISSN:0144-8463
1573-4935
DOI:10.1042/BSR20180457