Performance of the EUCAST disc diffusion method and two MIC methods in detection of Enterobacteriaceae with reduced susceptibility to meropenem: the NordicAST CPE study

To examine performance of EUCAST disc diffusion and supplementary MIC methods for detection of Enterobacteriaceae with reduced susceptibility to meropenem using EUCAST screening recommendations. Sixty-one Nordic laboratories delivered data on EUCAST disc diffusion (n = 61), semi-automated meropenem...

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Published in:Journal of antimicrobial chemotherapy 2018-10, Vol.73 (10), p.2738-2747
Main Authors: Haldorsen, Bjørg, Giske, Christian G, Hansen, Dennis S, Helgason, Kristjan Orri, Kahlmeter, Gunnar, Löhr, Iren H, Matuschek, Erika, Österblad, Monica, Rantakokko-Jalava, Kaisu, Wang, Mikala, Småbrekke, Lars, Samuelsen, Ørjan, Sundsfjord, Arnfinn
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Language:English
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Summary:To examine performance of EUCAST disc diffusion and supplementary MIC methods for detection of Enterobacteriaceae with reduced susceptibility to meropenem using EUCAST screening recommendations. Sixty-one Nordic laboratories delivered data on EUCAST disc diffusion (n = 61), semi-automated meropenem MIC (n = 23; VITEK2, n = 20 and Phoenix, n = 3) and gradient meropenem MIC (n = 58) methods. The strains (n = 27) included the major carbapenemase classes (A, n = 4; B, n = 9; D, n = 6) involved in the global spread of carbapenemase-producing Enterobacteriaceae (CPE) and non-CPE strains (n = 8) covering a range of broth microdilution (BMD) meropenem MICs. A triplicate Klebsiella variicola (meropenem MIC 0.5 mg/L) harbouring OXA-48 and Escherichia coli ATCC 25922 showed an overall good precision. Meropenem zone diameters below the EUCAST screening cut-off (
ISSN:0305-7453
1460-2091
1460-2091
DOI:10.1093/jac/dky276