Performance of the EUCAST disc diffusion method and two MIC methods in detection of Enterobacteriaceae with reduced susceptibility to meropenem: the NordicAST CPE study

To examine performance of EUCAST disc diffusion and supplementary MIC methods for detection of Enterobacteriaceae with reduced susceptibility to meropenem using EUCAST screening recommendations. Sixty-one Nordic laboratories delivered data on EUCAST disc diffusion (n = 61), semi-automated meropenem...

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Published in:Journal of antimicrobial chemotherapy 2018-10, Vol.73 (10), p.2738-2747
Main Authors: Haldorsen, Bjørg, Giske, Christian G, Hansen, Dennis S, Helgason, Kristjan Orri, Kahlmeter, Gunnar, Löhr, Iren H, Matuschek, Erika, Österblad, Monica, Rantakokko-Jalava, Kaisu, Wang, Mikala, Småbrekke, Lars, Samuelsen, Ørjan, Sundsfjord, Arnfinn
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cited_by cdi_FETCH-LOGICAL-c3176-8b2e296cfcb0b978f2434466278c7405fea56ec7fa55e34d81c23ff6d483601a3
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creator Haldorsen, Bjørg
Giske, Christian G
Hansen, Dennis S
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Kahlmeter, Gunnar
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Matuschek, Erika
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Rantakokko-Jalava, Kaisu
Wang, Mikala
Småbrekke, Lars
Samuelsen, Ørjan
Sundsfjord, Arnfinn
description To examine performance of EUCAST disc diffusion and supplementary MIC methods for detection of Enterobacteriaceae with reduced susceptibility to meropenem using EUCAST screening recommendations. Sixty-one Nordic laboratories delivered data on EUCAST disc diffusion (n = 61), semi-automated meropenem MIC (n = 23; VITEK2, n = 20 and Phoenix, n = 3) and gradient meropenem MIC (n = 58) methods. The strains (n = 27) included the major carbapenemase classes (A, n = 4; B, n = 9; D, n = 6) involved in the global spread of carbapenemase-producing Enterobacteriaceae (CPE) and non-CPE strains (n = 8) covering a range of broth microdilution (BMD) meropenem MICs. A triplicate Klebsiella variicola (meropenem MIC 0.5 mg/L) harbouring OXA-48 and Escherichia coli ATCC 25922 showed an overall good precision. Meropenem zone diameters below the EUCAST screening cut-off (
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title Performance of the EUCAST disc diffusion method and two MIC methods in detection of Enterobacteriaceae with reduced susceptibility to meropenem: the NordicAST CPE study
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