Extended photoacoustic transport model for characterization of red blood cell morphology in microchannel flow

• Published in: Biomedical optics express 2018-06, Vol.9 (6), p.2785-2809
• Main Authors: Uluc, Nasire, Unlu, Mehmet Burcin, Gulsen, Gultekin, Erkol, Hakan
• Format: Article
• Language: English
• ISSN: 2156-7085; 2156-7085
• DOI: 10.1364/BOE.9.002785

The dynamic response behavior of red blood cells holds the key to understanding red blood cell related diseases. In this regard, an understanding of the physiological functions of erythrocytes is significant before focusing on red blood cell aggregation in the microcirculatory system. In this work, we present a theoretical model for a photoacoustic signal that occurs when deformed red blood cells pass through a microfluidic channel. Using a Green's function approach, the photoacoustic pressure wave is obtained analytically by solving a combined Navier-Stokes and photoacoustic equation system. The photoacoustic wave expression includes determinant parameters for the cell deformability such as plasma viscosity, density, and red blood cell aggregation, as well as involving laser parameters such as beamwidth, pulse duration, and repetition rate. The effects of aggregation on blood rheology are also investigated. The results presented by this study show good agreements with the experimental ones in the literature. The comprehensive analytical solution of the extended photoacoustic transport model including a modified Morse type potential function sheds light on the dynamics of aggregate formation and demonstrates that the profile of a photoacoustic pressure wave has the potential for detecting and characterizing red blood cell aggregation.