Dissemination and persistence of extended-spectrum cephalosporin-resistance encoding IncI1-blaCTXM-1 plasmid among Escherichia coli in pigs

This study investigated the ecology, epidemiology and plasmid characteristics of extended-spectrum cephalosporin (ESC)-resistant E. coli in healthy pigs over a period of 4 years (2013–2016) following the withdrawal of ESCs. High carriage rates of ESC-resistant E. coli were demonstrated in 2013 (86.6...

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Bibliographic Details
Published in:The ISME Journal 2018-10, Vol.12 (10), p.2352-2362
Main Authors: Abraham, Sam, Kirkwood, Roy N., Laird, Tanya, Saputra, Sugiyono, Mitchell, Tahlia, Singh, Mohinder, Linn, Benjamin, Abraham, Rebecca J., Pang, Stanley, Gordon, David M., Trott, Darren J., O’Dea, Mark
Format: Article
Language:English
Subjects:
38/23
45/77
631/208/325/2482
631/326/22/1290
Biomedical and Life Sciences
Ecology
Evolutionary Biology
Life Sciences
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
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Summary:This study investigated the ecology, epidemiology and plasmid characteristics of extended-spectrum cephalosporin (ESC)-resistant E. coli in healthy pigs over a period of 4 years (2013–2016) following the withdrawal of ESCs. High carriage rates of ESC-resistant E. coli were demonstrated in 2013 (86.6%) and 2014 (83.3%), compared to 2015 (22%) and 2016 (8.5%). ESC resistance identified among E. coli isolates was attributed to the carriage of an IncI1 ST-3 plasmid (pCTXM1-MU2) encoding bla CTXM-1 . Genomic characterisation of selected E. coli isolates ( n  = 61) identified plasmid movement into multiple commensal E. coli ( n  = 22 STs). Major STs included ST10, ST5440, ST453, ST2514 and ST23. A subset of the isolates belong to the atypical enteropathogenic E. coli (aEPEC) pathotype that harboured multiple LEE pathogenic islands. pCTXM1-MU2 was similar (99% nt identity) to IncI1-ST3 plasmids reported from Europe, encoded resistance to aminoglycosides, sulphonamides and trimethoprim, and carried colicin Ib . pCTXM1-MU2 appears to be highly stable and readily transferable. This study demonstrates that ESC resistance may persist for a protracted period following removal of direct selection pressure, resulting in the emergence of ESC-resistance in both commensal E. coli and aEPEC isolates of potential significance to human and animal health.
ISSN:1751-7362
1751-7370
DOI:10.1038/s41396-018-0200-3