Saccharomyces cerevisiae Mhr1 can bind Xho I-induced mitochondrial DNA double-strand breaks in vivo

Mitochondrial DNA (mtDNA) double-strand break (DSB) repair is essential for maintaining mtDNA integrity, but little is known about the proteins involved in mtDNA DSB repair. Here, we utilize Saccharomyces cerevisiae as a eukaryotic model to identify proteins involved in mtDNA DSB repair. We show tha...

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Bibliographic Details
Published in:Mitochondrion 2018-09, Vol.42, p.23-32
Main Authors: Prasai, Kanchanjunga, Robinson, Lucy C, Tatchell, Kelly, Harrison, Lynn
Format: Article
Language:English
Subjects:
DNA Breaks, Double-Stranded
DNA Repair
DNA, Fungal - metabolism
DNA, Mitochondrial - metabolism
DNA-Binding Proteins - metabolism
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae Proteins - metabolism
Transcription Factors - metabolism
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Summary:Mitochondrial DNA (mtDNA) double-strand break (DSB) repair is essential for maintaining mtDNA integrity, but little is known about the proteins involved in mtDNA DSB repair. Here, we utilize Saccharomyces cerevisiae as a eukaryotic model to identify proteins involved in mtDNA DSB repair. We show that Mhr1, a protein known to possess homologous DNA pairing activity in vitro, binds to mtDNA DSBs in vivo, indicating its involvement in mtDNA DSB repair. Our data also indicate that Yku80, a protein previously implicated in mtDNA DSB repair, does not compete with Mhr1 for binding to mtDNA DSBs. In fact, C-terminally tagged Yku80 could not be detected in yeast mitochondrial extracts. Therefore, we conclude that Mhr1, but not Yku80, is a potential mtDNA DSB repair factor in yeast.
ISSN:1567-7249
1872-8278
DOI:10.1016/j.mito.2017.10.005