Nuclear expansion and pore opening are instant signs of neuronal hypoxia and can identify poorly fixed brains

The initial phase of neuronal death is not well characterized. Here, we show that expansion of the nuclear membrane without losing its integrity along with peripheralization of chromatin are immediate signs of neuronal injury. Importantly, these changes can be identified with commonly used nuclear s...

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Bibliographic Details
Published in:Scientific reports 2018-10, Vol.8 (1), p.14770-13, Article 14770
Main Authors: Dehghani, Anisa, Karatas, Hulya, Can, Alp, Erdemli, Esra, Yemisci, Muge, Eren-Kocak, Emine, Dalkara, Turgay
Format: Article
Language:English
Subjects:
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14/1
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631/80
Chromatin
HMGB1 protein
Humanities and Social Sciences
Hypoxia
Ischemia
Mitochondria
multidisciplinary
Neuroprotection
p53 Protein
Perfusion
Science
Science (multidisciplinary)
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Summary:The initial phase of neuronal death is not well characterized. Here, we show that expansion of the nuclear membrane without losing its integrity along with peripheralization of chromatin are immediate signs of neuronal injury. Importantly, these changes can be identified with commonly used nuclear stains and used as markers of poor perfusion-fixation. Although frozen sections are widely used, no markers are available to ensure that the observed changes were not confounded by perfusion-induced hypoxia/ischemia. Moreover, HMGB1 was immediately released and p53 translocated to mitochondria in hypoxic/ischemic neurons, whereas nuclear pore complex inhibitors prevented the nuclear changes, identifying novel neuroprotection targets.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-32878-1