LncRNA-MEG3 inhibits activation of hepatic stellate cells through SMO protein and miR-212

• Published in: Cell death & disease 2018-10, Vol.9 (10), p.1014-12, Article 1014
• Main Authors: Yu, Fujun, Geng, Wujun, Dong, Peihong, Huang, Zhiming, Zheng, Jianjian
• Format: Article
• Language: English
• Subjects: 13/51; 14; 14/19; 38; 38/77; 38/90; 64/60; 82/80; 96/95; Animals; Antibodies; Bile; Biochemistry; Biomarkers - metabolism; Biomedical and Life Sciences; Case-Control Studies; Cell activation; Cell Biology; Cell Culture; Cell Proliferation - genetics; Clonal deletion; Collagen (type I); Collagen Type I - genetics; Epithelial Cells - pathology; Epithelial-Mesenchymal Transition - genetics; Fibrosis; Hedgehog protein; Hedgehog Proteins - genetics; Hepatic Stellate Cells - physiology; Hepatitis B; Humans; Immunology; Immunoprecipitation; Kinases; Life Sciences; Liver; Liver - physiology; Liver Cirrhosis - genetics; Liver Cirrhosis - pathology; Male; Mesenchyme; Mice; Mice, Inbred C57BL; MicroRNAs - genetics; miRNA; Non-coding RNA; Proteins; RNA, Long Noncoding - genetics; Smoothened Receptor - genetics; Stellate cells; Tumor suppressor genes; Tumors
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/s41419-018-1068-x

Activation of hepatic stellate cells (HSCs), a pivotal event in liver fibrosis, is considered as an epithelial–mesenchymal transition (EMT) process. Deregulation of long noncoding RNAs (lncRNAs) has been reported to be involved in a series of human diseases. LncRNA-maternally expressed gene 3 (MEG3) functions as a tumor suppressor in cancers and has been shown to play a vital role in EMT process. However, the biological role of MEG3 in liver fibrosis is largely unknown. In this study, MEG3 was reduced in vivo and in vitro during liver fibrosis. Restoring of MEG3 expression led to the suppression of liver fibrosis, with a reduction in α-SMA and type I collagen. Notably, MEG3 overexpression inhibited HSC activation through EMT, associated with an increase in epithelial markers and a reduction in mesenchymal markers. Further studies showed that Hedgehog (Hh) pathway-mediated EMT process was involved in the effects of MEG3 on HSC activation. Smoothened (SMO) is a member of Hh pathway. Using bioinformatic analysis, an interaction between MEG3 and SMO protein was predicted. This interaction was confirmed by the results of RNA immunoprecipitation and deletion-mapping analysis. Furthermore, MEG3 was confirmed as a target of microRNA-212 (miR-212). miR-212 was partly responsible for the effects of MEG3 on EMT process. Interestingly, MEG3 was also reduced in chronic hepatitis B (CHB) patients with liver fibrosis when compared with healthy controls. MEG3 negatively correlated with fibrosis stage in CHB patients. In conclusion, we demonstrate that MEG3 inhibits Hh-mediated EMT process in liver fibrosis via SMO protein and miR-212.