Loading…

LINC00152 Promotes Invasion through a 3'-Hairpin Structure and Associates with Prognosis in Glioblastoma

Long noncoding RNAs (lncRNA) are increasingly implicated in oncogenesis. Here, it is determined that / is upregulated in glioblastoma multiforme (GBM) and aggressive wild-type IDH1/2 grade 2/3 gliomas and upregulation associates with poor patient outcomes. is similarly upregulated in over 10 other c...

Full description

Saved in:
Bibliographic Details
Published in:Molecular cancer research 2018-10, Vol.16 (10), p.1470-1482
Main Authors: Reon, Brian J, Takao Real Karia, Bruno, Kiran, Manjari, Dutta, Anindya
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Long noncoding RNAs (lncRNA) are increasingly implicated in oncogenesis. Here, it is determined that / is upregulated in glioblastoma multiforme (GBM) and aggressive wild-type IDH1/2 grade 2/3 gliomas and upregulation associates with poor patient outcomes. is similarly upregulated in over 10 other cancer types and associates with a poor prognosis in 7 other cancer types. Inhibition of the mostly cytoplasmic decreases, and overexpression increases cellular invasion. knockdown alters the transcription of genes important to epithelial-to-mesenchymal transition (EMT). PARIS and Ribo-seq data, together with secondary structure prediction, identified a protein-bound 121-bp stem-loop structure at the 3' end of whose overexpression is sufficient to increase invasion of GBM cells. Point mutations in the stem-loop suggest that stem formation in the hairpin is essential for function. has a nearly identical homolog, , which encodes a near identical hairpin, is equally expressed in low-grade glioma (LGG) and GBM, predicts poor patient survival in these tumors, and is also reduced by knockdown. Together, these data reveal that and its homolog associate with aggressive tumors and promote cellular invasion through a mechanism that requires the structural integrity of a hairpin structure. Frequent upregulation of the lncRNA, , in glioblastoma and other tumor types combined with its prognostic potential and ability to promote invasion suggests as a potential biomarker and therapeutic target. .
ISSN:1541-7786
1557-3125
DOI:10.1158/1541-7786.MCR-18-0322