Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships

• Published in: Cell 2018-09, Vol.174 (6), p.1450-1464.e23
• Main Authors: Zhang, Peng, Lu, Hong, Peixoto, Rui T., Pines, Mary K., Ge, Yuan, Oku, Shinichiro, Siddiqui, Tabrez J., Xie, Yicheng, Wu, Wenlan, Archer-Hartmann, Stephanie, Yoshida, Keitaro, Tanaka, Kenji F., Aricescu, A. Radu, Azadi, Parastoo, Gordon, Michael D., Sabatini, Bernardo L., Wong, Rachel O.L., Craig, Ann Marie
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Calcium-Binding Proteins; Cell Adhesion Molecules, Neuronal - antagonists & inhibitors; Cell Adhesion Molecules, Neuronal - genetics; Cell Adhesion Molecules, Neuronal - metabolism; Drosophila; Drosophila Proteins - antagonists & inhibitors; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Female; Glycopeptides - analysis; heparan sulphate; Heparitin Sulfate - chemistry; Heparitin Sulfate - metabolism; Humans; LRRTM; Membrane Proteins; Mice; Mice, Inbred C57BL; mossy fiber; Nerve Tissue Proteins; Neural Cell Adhesion Molecules - antagonists & inhibitors; Neural Cell Adhesion Molecules - genetics; Neural Cell Adhesion Molecules - metabolism; neurexin; neuroligin; Neurons - cytology; Neurons - metabolism; Protein Binding; proteoglycan; Rats; RNA Interference; RNA, Small Interfering - metabolism; Sequence Alignment; Synapses - metabolism; synaptic adhesion protein; synaptic transmission; synaptogenesis; thorny excrescence
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/j.cell.2018.07.002

Synapses are fundamental units of communication in the brain. The prototypical synapse-organizing complex neurexin-neuroligin mediates synapse development and function and is central to a shared genetic risk pathway in autism and schizophrenia. Neurexin’s role in synapse development is thought to be mediated purely by its protein domains, but we reveal a requirement for a rare glycan modification. Mice lacking heparan sulfate (HS) on neurexin-1 show reduced survival, as well as structural and functional deficits at central synapses. HS directly binds postsynaptic partners neuroligins and LRRTMs, revealing a dual binding mode involving intrinsic glycan and protein domains for canonical synapse-organizing complexes. Neurexin HS chains also bind novel ligands, potentially expanding the neurexin interactome to hundreds of HS-binding proteins. Because HS structure is heterogeneous, our findings indicate an additional dimension to neurexin diversity, provide a molecular basis for fine-tuning synaptic function, and open therapeutic directions targeting glycan-binding motifs critical for brain development. [Display omitted] •Brain central synaptic organizers neurexins are heparan sulfate (HS) proteoglycans•A dual HS and protein domain mode mediates neurexin binding to neuroligin and LRRTM•HS increases neurexin heterogeneity and expands its interactome to HS binding factors•HS glycan on neurexin is required for normal synaptic development and mouse survival Neurexins, major synaptic-organizing proteins, are heparan sulfate (HS) proteoglycans, and HS modification is required for neurexin functions in synaptic transmission, development, and behavior.