Puerarin 6″-O-xyloside possesses significant antitumor activities on colon cancer through inducing apoptosis

• Published in: Oncology letters 2018-11, Vol.16 (5), p.5557-5564
• Main Authors: Zhang, Xiao-Lan, Wang, Bin-Bin, Mo, Jian-Shu
• Format: Article
• Language: English
• Subjects: Alzheimer's disease; Apoptosis; Biotechnology; Cancer therapies; Cell growth; Colorectal cancer; Cytochrome; Cytotoxicity; Kinases; Lymphoma; Metastasis; Mitochondria; Oncology; Proteins; Tumors; Vascular endothelial growth factor
• ISSN: 1792-1074; 1792-1082
• DOI: 10.3892/ol.2018.9364

Puerarin 6″-O-xyloside (PRX) is a major compound found in the root of the (Willd.) Ohwi. The present study aimed to investigate the antitumor activity of PRX against colon cancer and examine its possible mechanism. In the present study, the anti-proliferative effects of PRX against colon cell lines (SW480, LoVo and HCT-116) were evaluated using a Cell Counting Kit-8 assay, and the half maximal inhibitory concentration values of the SW480, LoVo and HCT-11 cells were 37.114, 49.213 and 43.022 µg/ml, respectively. Furthermore, the apoptosis of SW480 cells was detected using flow cytometry with Annexin V-fluorescein isothiocyanate/propidium iodide staining. Subsequently, western blot analysis was performed to examine the expression of proteins associated with apoptosis, invasion and metastasis of tumors. The results showed that PRX possessed antitumor activity against colon cancer cell lines in a dose-dependent and time-dependent manner. In addition, PRX significantly upregulated the expression levels of cleaved (c)-caspase-3, c-caspase-9, B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein and phosphorylated c-Jun terminal kinase, and downregulated the expression levels of Bcl-2, matrix metalloproteinase (MMP)-3, MMP-9 and vascular endothelial growth factor (P