Amelioration of arsenic-induced oxidative stress in CHO cells by Ixora coccinea flower extract

Chronic exposure to inorganic arsenic creates various health problems. Ixora coccinea flower extract was investigated for its ability to protect against arsenic-induced cytotoxicity and genotoxicity in CHO cell line. MTT assay confirmed the efficacy of the extract in ameliorating arsenic-induced cyt...

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Bibliographic Details
Published in:3 Biotech 2018-10, Vol.8 (10), p.446-7, Article 446
Main Authors: Salmataj, S. A., Kamath, Shobha U., Murty, V. Ramachandra, Pai, Sreedhara Ranganath
Format: Article
Language:English
Subjects:
Agriculture
Antioxidants
Apoptosis
Arsenates
Arsenic
Assaying
Bioinformatics
Biomaterials
Biotechnology
Cancer Research
Catalase
Chemistry
Chemistry and Materials Science
Chronic exposure
Cytotoxicity
Deoxyribonucleic acid
Depolarization
DNA
DNA damage
Flowers
Free radicals
Genotoxicity
Ixora coccinea
Membrane potential
Mitochondria
Nitric oxide
Original
Original Article
Oxidative stress
Pretreatment
Reactive oxygen species
Scavenging
Sodium arsenate
Stem Cells
Superoxide dismutase
Toxicity
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Summary:Chronic exposure to inorganic arsenic creates various health problems. Ixora coccinea flower extract was investigated for its ability to protect against arsenic-induced cytotoxicity and genotoxicity in CHO cell line. MTT assay confirmed the efficacy of the extract in ameliorating arsenic-induced cytotoxicity. The value (48 mM) of 24 h inhibitory concentration (IC50) of sodium arsenate for CHO cells was obtained by MTT assay. Various free radical scavenging assays like DPPH, ABTS and nitric oxide scavenging assay confirmed antioxidant activity of the Ixora coccinea flower extract. Pretreatment of the extract significantly inhibited the arsenic-induced DNA damage ( p  
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-018-1446-1