Multiplex Biomarker Screening Assay for Urinary Extracellular Vesicles Study: A Targeted Label-Free Proteomic Approach

• Published in: Scientific reports 2018-10, Vol.8 (1), p.15039-8, Article 15039
• Main Authors: Chutipongtanate, Somchai, Greis, Kenneth D.
• Format: Article
• Language: English
• Subjects: 631/61/475; 692/4022; 82/1; 82/29; 82/58; Biomarkers; Biomarkers - metabolism; Calcium-Binding Proteins - metabolism; CD63 antigen; CD9 antigen; Cell Cycle Proteins - metabolism; Cell-Derived Microparticles - genetics; Cell-Derived Microparticles - metabolism; Coefficient of variation; Data processing; DNA-Binding Proteins - metabolism; Endosomal Sorting Complexes Required for Transport - metabolism; Exosomes - genetics; Exosomes - metabolism; Extracellular vesicles; Extracellular Vesicles - genetics; Extracellular Vesicles - metabolism; Hsp70 protein; HSP72 Heat-Shock Proteins - metabolism; Humanities and Social Sciences; Humans; multidisciplinary; Proteins; Proteomics; Quantitation; Science; Science (multidisciplinary); Tandem Mass Spectrometry; Tetraspanin 29 - metabolism; Tetraspanin 30 - metabolism; Transcription Factors - metabolism; Urinary Tract - metabolism; Urine
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-33280-7

The recent advance in targeted label-free proteomics, SWATH-MS, can provide consistent protein detection and reproducible protein quantitation, which is a considerable advantage for biomarker study of urinary extracellular vesicles. We developed a SWATH-MS workflow with a curated spectral library of 1,145 targets. Application of the workflow across nine replicates of three sample types (exosome-like vesicles (ELVs), microvesicles (MVs) and urine proteins (UPs)) resulted in the quantitation of 888 proteins at FDR