Optofluidic detection of Zika nucleic acid and protein biomarkers using multimode interference multiplexing

The recent massive Zika virus (ZIKV) outbreak illustrates the need for rapid and specific diagnostic techniques. Detecting ZIKV in biological samples poses unique problems: antibody detection of ZIKV is insufficient due to cross-reactivity of Zika antibodies with other flaviviruses, and nucleic acid...

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Bibliographic Details
Published in:Biomedical optics express 2018-08, Vol.9 (8), p.3725-3730
Main Authors: Stambaugh, Alexandra, Parks, Joshua W, Stott, Matthew A, Meena, Gopikrishnan G, Hawkins, Aaron R, Schmidt, Holger
Format: Article
Language:English
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Summary:The recent massive Zika virus (ZIKV) outbreak illustrates the need for rapid and specific diagnostic techniques. Detecting ZIKV in biological samples poses unique problems: antibody detection of ZIKV is insufficient due to cross-reactivity of Zika antibodies with other flaviviruses, and nucleic acid and protein biomarkers for ZIKV are detectable at different stages of infection. Here, we describe a new optofluidic approach for the parallel detection of different molecular biomarkers using multimode interference (MMI) waveguides. We report differentiated, multiplex detection of both ZIKV biomarker types using multi-spot excitation at two visible wavelengths with over 98% fidelity by combining several analysis techniques.
ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.9.003725