Community dynamics and functional characteristics of naphthalene‐degrading populations in contaminated surface sediments and hypoxic/anoxic groundwater

• Published in: Environmental microbiology 2018-10, Vol.20 (10), p.3543-3559
• Main Authors: Wilhelm, Roland C., Hanson, Buck T., Chandra, Subhash, Madsen, Eugene
• Format: Article
• Language: English
• Subjects: Anoxia; Attenuation; Bacteria; Bacteria - genetics; Bacteria - metabolism; Biodegradation; Biodegradation, Environmental; Biogeochemistry; Carbon; Catabolism; Coal tar; Contaminants; Contamination; Cultivation; Degradation; Dynamics; Feeders; Genomes; Groundwater; Groundwater - microbiology; Groundwater pollution; Hypoxia; Imaging techniques; Interactions; Mass spectrometry; Mass spectroscopy; Metabolism; Metagenome; Metagenomics; Microbial contamination; Microorganisms; Naphthalene; Naphthalenes - metabolism; Natural attenuation; Populations; Protozoa; Rhodococcus; Secondary ion mass spectrometry; Sediment; Sediment pollution; Sediments; Soil Microbiology; Soil Pollutants - metabolism; Stable isotopes; Stenotrophomonas; Trophic relationships; Water Microbiology; Water Pollutants, Chemical - metabolism
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.14309

Summary Earlier research on the biogeochemical factors affecting natural attenuation in coal‐tar contaminated groundwater, at South Glens Falls, NY, revealed the importance of anaerobic metabolism and trophic interactions between degrader and bacterivore populations. Field‐based characterizations of both phenomena have proven challenging, but advances in stable isotope probing (SIP), single‐cell imaging and shotgun metagenomics now provide cultivation‐independent tools for their study. We tracked carbon from 13C‐labelled naphthalene through microbial populations in contaminated surface sediments over 6 days using respiration assays, secondary ion mass spectrometry imaging and shotgun metagenomics to disentangle the contaminant‐based trophic web. Contaminant‐exposed communities in hypoxic/anoxic groundwater were contrasted with those from oxic surface sediments to identify putative features of anaerobic catabolism of naphthalene. In total, six bacteria were responsible for naphthalene degradation. Cupriavidus, Ralstonia and Sphingomonas predominated at the earliest stages of SIP incubations and were succeeded in later stages by Stenotrophomonas and Rhodococcus. Metagenome‐assembled genomes provided evidence for the ecological and functional characteristics underlying these temporal shifts. Identical species of Stenotrophomonas and Rhodococcus were abundant in the most contaminated, anoxic groundwater. Apparent increases in bacterivorous protozoa were observed following exposure to naphthalene, though insignificant amounts of carbon were transferred between bacterial degraders and populations of secondary feeders.