Whole genome amplification and exome sequencing of archived schistosome miracidia

Adult schistosomes live in the blood vessels and cannot easily be sampled from humans, so archived miracidia larvae hatched from eggs expelled in feces or urine are commonly used for population genetic studies. Large collections of archived miracidia on FTA cards are now available through the Schist...

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Bibliographic Details
Published in:Parasitology 2018-11, Vol.145 (13), p.1739-1747
Main Authors: Le Clec'h, Winka, Chevalier, Frédéric D., McDew-White, Marina, Allan, Fiona, Webster, Bonnie L., Gouvras, Anouk N., Kinunghi, Safari, Tchuem Tchuenté, Louis-Albert, Garba, Amadou, Mohammed, Khalfan A., Ame, Shaali M., Webster, Joanne P., Rollinson, David, Emery, Aidan M., Anderson, Timothy J. C.
Format: Article
Language:English
Subjects:
Adults
Amplification
Animals
Biological Specimen Banks
Biomedical research
Blood vessels
Child
Data collection
Deoxyribonucleic acid
DNA
DNA sequencing
DNA, Helminth - genetics
Eggs
Ethics
Exome Sequencing
Feces - parasitology
Female
Gene polymorphism
Genetics
Genome, Helminth
Genomes
Genomics
Grants
High-Throughput Nucleotide Sequencing
Humans
Larvae
Library collections
Male
Medical research
Methods
Natural history
Nucleic Acid Amplification Techniques
Polymerase Chain Reaction
Polymorphism
Polymorphism, Genetic
Population genetics
Population studies
Schistosoma haematobium - genetics
Schistosoma mansoni - genetics
Schistosomiasis
Special Issue Research Article
Tropical diseases
Urine
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Summary:Adult schistosomes live in the blood vessels and cannot easily be sampled from humans, so archived miracidia larvae hatched from eggs expelled in feces or urine are commonly used for population genetic studies. Large collections of archived miracidia on FTA cards are now available through the Schistosomiasis Collection at the Natural History Museum (SCAN). Here we describe protocols for whole genome amplification of Schistosoma mansoni and Schistosome haematobium miracidia from these cards, as well as real time PCR quantification of amplified schistosome DNA. We used microgram quantities of DNA obtained for exome capture and sequencing of single miracidia, generating dense polymorphism data across the exome. These methods will facilitate the transition from population genetics, using limited numbers of markers to population genomics using genome-wide marker information, maximising the value of collections such as SCAN.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182018000811