Upregulation of miR-200b Inhibits Hepatocellular Carcinoma Cell Proliferation and Migration by Targeting HMGB3 Protein

HMGB3 belongs to the high-mobility group box subfamily and has been found to be overexpressed in gastric cancer. However, the expression and the role of HMGB3 in human hepatocellular carcinoma remain unknown. Here, we report that HMGB3, which is suppressed by miR-200b, contributes to cell proliferat...

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Published in:Technology in cancer research & treatment 2018-01, Vol.17, p.1533033818806475-1533033818806475
Main Authors: Wang, Long-kun, Xie, Xi-Na, Song, Xu-Hong, Su, Ting, Chang, Xiao-Lan, Xu, Man, Liang, Bin, Huang, Dong-Yang
Format: Article
Language:English
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Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - mortality
Carcinoma, Hepatocellular - pathology
Cell growth
Cell Line, Tumor
Cell Movement - genetics
Cell Proliferation - genetics
Disease Progression
Gene Expression Regulation, Neoplastic
HMGB3 Protein - genetics
Humans
Kaplan-Meier Estimate
Liver cancer
Liver Neoplasms - genetics
Liver Neoplasms - mortality
Liver Neoplasms - pathology
MicroRNAs - genetics
Original
Prognosis
RNA Interference
Up-regulation
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Summary:HMGB3 belongs to the high-mobility group box subfamily and has been found to be overexpressed in gastric cancer. However, the expression and the role of HMGB3 in human hepatocellular carcinoma remain unknown. Here, we report that HMGB3, which is suppressed by miR-200b, contributes to cell proliferation and migration in human hepatocellular carcinoma. After analyzing The Cancer Genome Atlas data of 371 patients with hepatocellular carcinoma, we identified HMGB3 to be upregulated in human hepatocellular carcinoma tissue. Knockdown of HMGB3 in the hepatocellular carcinoma cell line suppressed cell proliferation and migration. TargetScan analysis showed miR-200b to be a possible regulator for HMGB3. Subsequent luciferase assays indicated that HMGB3 was a direct target of miR-200b. In addition, upregulation of miR-200b inhibited hepatocellular carcinoma cell growth and migration. HMGB3 overexpression or miR-200b downregulation was associated with poor prognosis. Our findings suggest HMGB3 may serve as an important oncoprotein whose expression is negatively regulated by miR-200b in hepatocellular carcinoma.
ISSN:1533-0346
1533-0338
DOI:10.1177/1533033818806475