Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing

Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and contro...

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Bibliographic Details
Published in:Scientific reports 2018-10, Vol.8 (1), p.15869-12, Article 15869
Main Authors: Na, Jongbeom, Song, Seung Yong, Kim, Jae Dong, Han, Minsu, Heo, June Seok, Yang, Chae Eun, Kim, Hyun Ok, Lew, Dae Hyun, Kim, Eunkyoung
Format: Article
Language:English
Subjects:
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Cell adhesion
Cell proliferation
Collagen
Diabetes mellitus
Fibronectin
Harvesting
Humanities and Social Sciences
multidisciplinary
Polymers
Science
Science (multidisciplinary)
Stem cell transplantation
Stem cells
Wound healing
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Summary:Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and control both cell-to-cell and cell-to-substrate interactions. Through this engineering of FN, a transferrable hADSC sheet was obtained as a free-stranding sheet (122.6 mm 2 ) by a photothermal method. During the harvesting of hADSC sheets by the photothermal method, a collagen layer in-between cells and conductive polymer film (CP) was dissociated, to protect cells from direct exposure to a near infrared (NIR) source. The hADSC sheets were applied to chronic wound of genetically diabetic db / db mice in vivo , to accelerate 30% faster wound closure with a high closure effect (ε wc ) than that of control groups. These results indicated that the engineering of CAM and collagens allow hADSC sheet harvesting, which could be extended to engineer various stem cell sheets for efficient therapies.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-34119-x