Loading…
Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing
Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and contro...
Saved in:
| Published in: | Scientific reports 2018-10, Vol.8 (1), p.15869-12, Article 15869 |
|---|---|
| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c577t-1699d3ed0007980fe7a269818beb2d73f4cbc3c5fc4191f8f310c457f32dafe83 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c577t-1699d3ed0007980fe7a269818beb2d73f4cbc3c5fc4191f8f310c457f32dafe83 |
| container_end_page | 12 |
| container_issue | 1 |
| container_start_page | 15869 |
| container_title | Scientific reports |
| container_volume | 8 |
| creator | Na, Jongbeom Song, Seung Yong Kim, Jae Dong Han, Minsu Heo, June Seok Yang, Chae Eun Kim, Hyun Ok Lew, Dae Hyun Kim, Eunkyoung |
| description | Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and control both cell-to-cell and cell-to-substrate interactions. Through this engineering of FN, a transferrable hADSC sheet was obtained as a free-stranding sheet (122.6 mm
2
) by a photothermal method. During the harvesting of hADSC sheets by the photothermal method, a collagen layer in-between cells and conductive polymer film (CP) was dissociated, to protect cells from direct exposure to a near infrared (NIR) source. The hADSC sheets were applied to chronic wound of genetically diabetic
db
/
db
mice
in vivo
, to accelerate 30% faster wound closure with a high closure effect (ε
wc
) than that of control groups. These results indicated that the engineering of CAM and collagens allow hADSC sheet harvesting, which could be extended to engineer various stem cell sheets for efficient therapies. |
| doi_str_mv | 10.1038/s41598-018-34119-x |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6203842</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2126917272</sourcerecordid><originalsourceid>FETCH-LOGICAL-c577t-1699d3ed0007980fe7a269818beb2d73f4cbc3c5fc4191f8f310c457f32dafe83</originalsourceid><addsrcrecordid>eNp9Uc9rFDEYDWKxpe0_4EEGvHhJzY-ZSXIRlqVaYcFCFY8hm3yZpswmazJT6n9vtltr24O5JPDe97738hB6S8kZJVx-LC3tlMSESsxbShW-e4WOGGk7zDhjr5-8D9FpKTekno6plqo36JAT3gui5BG6vMxpghDxeRxCBMjgmpXJAzSLDKZZuLBNBbCDHG4rdDXBplnCODZX1wBTaXzKzc80R9dcgBlDHE7QgTdjgdOH-xj9-Hz-fXmBV9--fF0uVth2QkyY9ko5Dq66EkoSD8KwXkkq17BmTnDf2rXltvO2OqZeek6JbTvhOXPGg-TH6NNedzuvN-AsxCmbUW9z2Jj8WycT9HMkhms9pFvds_p9LasCHx4Ecvo1Q5n0JhRbo5kIaS6a0WqICiZ21PcvqDdpzrHG27G6vlWc7RyxPcvmVEoG_2iGEr3rTO8707Uzfd-ZvqtD757GeBz521Al8D2hVCgOkP_t_o_sH8RKojI</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2125649328</pqid></control><display><type>article</type><title>Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing</title><source>PubMed (Medline)</source><source>ProQuest - Publicly Available Content Database</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature - nature.com Journals - Fully Open Access</source><creator>Na, Jongbeom ; Song, Seung Yong ; Kim, Jae Dong ; Han, Minsu ; Heo, June Seok ; Yang, Chae Eun ; Kim, Hyun Ok ; Lew, Dae Hyun ; Kim, Eunkyoung</creator><creatorcontrib>Na, Jongbeom ; Song, Seung Yong ; Kim, Jae Dong ; Han, Minsu ; Heo, June Seok ; Yang, Chae Eun ; Kim, Hyun Ok ; Lew, Dae Hyun ; Kim, Eunkyoung</creatorcontrib><description>Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and control both cell-to-cell and cell-to-substrate interactions. Through this engineering of FN, a transferrable hADSC sheet was obtained as a free-stranding sheet (122.6 mm
2
) by a photothermal method. During the harvesting of hADSC sheets by the photothermal method, a collagen layer in-between cells and conductive polymer film (CP) was dissociated, to protect cells from direct exposure to a near infrared (NIR) source. The hADSC sheets were applied to chronic wound of genetically diabetic
db
/
db
mice
in vivo
, to accelerate 30% faster wound closure with a high closure effect (ε
wc
) than that of control groups. These results indicated that the engineering of CAM and collagens allow hADSC sheet harvesting, which could be extended to engineer various stem cell sheets for efficient therapies.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-34119-x</identifier><identifier>PMID: 30367098</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/107 ; 631/532 ; 639/301/54 ; 82 ; Cell adhesion ; Cell proliferation ; Collagen ; Diabetes mellitus ; Fibronectin ; Harvesting ; Humanities and Social Sciences ; multidisciplinary ; Polymers ; Science ; Science (multidisciplinary) ; Stem cell transplantation ; Stem cells ; Wound healing</subject><ispartof>Scientific reports, 2018-10, Vol.8 (1), p.15869-12, Article 15869</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c577t-1699d3ed0007980fe7a269818beb2d73f4cbc3c5fc4191f8f310c457f32dafe83</citedby><cites>FETCH-LOGICAL-c577t-1699d3ed0007980fe7a269818beb2d73f4cbc3c5fc4191f8f310c457f32dafe83</cites><orcidid>0000-0001-8128-791X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2125649328/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2125649328?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30367098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Na, Jongbeom</creatorcontrib><creatorcontrib>Song, Seung Yong</creatorcontrib><creatorcontrib>Kim, Jae Dong</creatorcontrib><creatorcontrib>Han, Minsu</creatorcontrib><creatorcontrib>Heo, June Seok</creatorcontrib><creatorcontrib>Yang, Chae Eun</creatorcontrib><creatorcontrib>Kim, Hyun Ok</creatorcontrib><creatorcontrib>Lew, Dae Hyun</creatorcontrib><creatorcontrib>Kim, Eunkyoung</creatorcontrib><title>Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and control both cell-to-cell and cell-to-substrate interactions. Through this engineering of FN, a transferrable hADSC sheet was obtained as a free-stranding sheet (122.6 mm
2
) by a photothermal method. During the harvesting of hADSC sheets by the photothermal method, a collagen layer in-between cells and conductive polymer film (CP) was dissociated, to protect cells from direct exposure to a near infrared (NIR) source. The hADSC sheets were applied to chronic wound of genetically diabetic
db
/
db
mice
in vivo
, to accelerate 30% faster wound closure with a high closure effect (ε
wc
) than that of control groups. These results indicated that the engineering of CAM and collagens allow hADSC sheet harvesting, which could be extended to engineer various stem cell sheets for efficient therapies.</description><subject>13</subject><subject>13/107</subject><subject>631/532</subject><subject>639/301/54</subject><subject>82</subject><subject>Cell adhesion</subject><subject>Cell proliferation</subject><subject>Collagen</subject><subject>Diabetes mellitus</subject><subject>Fibronectin</subject><subject>Harvesting</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Polymers</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Wound healing</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNp9Uc9rFDEYDWKxpe0_4EEGvHhJzY-ZSXIRlqVaYcFCFY8hm3yZpswmazJT6n9vtltr24O5JPDe97738hB6S8kZJVx-LC3tlMSESsxbShW-e4WOGGk7zDhjr5-8D9FpKTekno6plqo36JAT3gui5BG6vMxpghDxeRxCBMjgmpXJAzSLDKZZuLBNBbCDHG4rdDXBplnCODZX1wBTaXzKzc80R9dcgBlDHE7QgTdjgdOH-xj9-Hz-fXmBV9--fF0uVth2QkyY9ko5Dq66EkoSD8KwXkkq17BmTnDf2rXltvO2OqZeek6JbTvhOXPGg-TH6NNedzuvN-AsxCmbUW9z2Jj8WycT9HMkhms9pFvds_p9LasCHx4Ecvo1Q5n0JhRbo5kIaS6a0WqICiZ21PcvqDdpzrHG27G6vlWc7RyxPcvmVEoG_2iGEr3rTO8707Uzfd-ZvqtD757GeBz521Al8D2hVCgOkP_t_o_sH8RKojI</recordid><startdate>20181026</startdate><enddate>20181026</enddate><creator>Na, Jongbeom</creator><creator>Song, Seung Yong</creator><creator>Kim, Jae Dong</creator><creator>Han, Minsu</creator><creator>Heo, June Seok</creator><creator>Yang, Chae Eun</creator><creator>Kim, Hyun Ok</creator><creator>Lew, Dae Hyun</creator><creator>Kim, Eunkyoung</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8128-791X</orcidid></search><sort><creationdate>20181026</creationdate><title>Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing</title><author>Na, Jongbeom ; Song, Seung Yong ; Kim, Jae Dong ; Han, Minsu ; Heo, June Seok ; Yang, Chae Eun ; Kim, Hyun Ok ; Lew, Dae Hyun ; Kim, Eunkyoung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c577t-1699d3ed0007980fe7a269818beb2d73f4cbc3c5fc4191f8f310c457f32dafe83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>13</topic><topic>13/107</topic><topic>631/532</topic><topic>639/301/54</topic><topic>82</topic><topic>Cell adhesion</topic><topic>Cell proliferation</topic><topic>Collagen</topic><topic>Diabetes mellitus</topic><topic>Fibronectin</topic><topic>Harvesting</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Polymers</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Na, Jongbeom</creatorcontrib><creatorcontrib>Song, Seung Yong</creatorcontrib><creatorcontrib>Kim, Jae Dong</creatorcontrib><creatorcontrib>Han, Minsu</creatorcontrib><creatorcontrib>Heo, June Seok</creatorcontrib><creatorcontrib>Yang, Chae Eun</creatorcontrib><creatorcontrib>Kim, Hyun Ok</creatorcontrib><creatorcontrib>Lew, Dae Hyun</creatorcontrib><creatorcontrib>Kim, Eunkyoung</creatorcontrib><collection>SpringerOpen</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest - Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Na, Jongbeom</au><au>Song, Seung Yong</au><au>Kim, Jae Dong</au><au>Han, Minsu</au><au>Heo, June Seok</au><au>Yang, Chae Eun</au><au>Kim, Hyun Ok</au><au>Lew, Dae Hyun</au><au>Kim, Eunkyoung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-10-26</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>15869</spage><epage>12</epage><pages>15869-12</pages><artnum>15869</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and control both cell-to-cell and cell-to-substrate interactions. Through this engineering of FN, a transferrable hADSC sheet was obtained as a free-stranding sheet (122.6 mm
2
) by a photothermal method. During the harvesting of hADSC sheets by the photothermal method, a collagen layer in-between cells and conductive polymer film (CP) was dissociated, to protect cells from direct exposure to a near infrared (NIR) source. The hADSC sheets were applied to chronic wound of genetically diabetic
db
/
db
mice
in vivo
, to accelerate 30% faster wound closure with a high closure effect (ε
wc
) than that of control groups. These results indicated that the engineering of CAM and collagens allow hADSC sheet harvesting, which could be extended to engineer various stem cell sheets for efficient therapies.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30367098</pmid><doi>10.1038/s41598-018-34119-x</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-8128-791X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2018-10, Vol.8 (1), p.15869-12, Article 15869 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6203842 |
| source | PubMed (Medline); ProQuest - Publicly Available Content Database; Free Full-Text Journals in Chemistry; Springer Nature - nature.com Journals - Fully Open Access |
| subjects | 13 13/107 631/532 639/301/54 82 Cell adhesion Cell proliferation Collagen Diabetes mellitus Fibronectin Harvesting Humanities and Social Sciences multidisciplinary Polymers Science Science (multidisciplinary) Stem cell transplantation Stem cells Wound healing |
| title | Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T03%3A58%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protein-Engineered%20Large%20Area%20Adipose-derived%20Stem%20Cell%20Sheets%20for%20Wound%20Healing&rft.jtitle=Scientific%20reports&rft.au=Na,%20Jongbeom&rft.date=2018-10-26&rft.volume=8&rft.issue=1&rft.spage=15869&rft.epage=12&rft.pages=15869-12&rft.artnum=15869&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-018-34119-x&rft_dat=%3Cproquest_pubme%3E2126917272%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c577t-1699d3ed0007980fe7a269818beb2d73f4cbc3c5fc4191f8f310c457f32dafe83%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2125649328&rft_id=info:pmid/30367098&rfr_iscdi=true |