Loading…
Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin
Spider dragline silk is a remarkable material that shows excellent mechanical properties, diverse applications, biocompatibility and biodegradability. Transgenic silkworm technology was used to obtain four types of chimeric silkworm/spider (termed composite) silk fibres, including different lengths...
Saved in:
| Published in: | Scientific reports 2018-10, Vol.8 (1), p.15956-14, Article 15956 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c511t-5daa7541d5ddef41ab9ec3263ddcd489d40f33179948fd85ea1151932b6b528f3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c511t-5daa7541d5ddef41ab9ec3263ddcd489d40f33179948fd85ea1151932b6b528f3 |
| container_end_page | 14 |
| container_issue | 1 |
| container_start_page | 15956 |
| container_title | Scientific reports |
| container_volume | 8 |
| creator | You, Zhengying Ye, Xiaogang Ye, Lupeng Qian, Qiujie Wu, Meiyu Song, Jia Che, Jiaqian Zhong, Boxiong |
| description | Spider dragline silk is a remarkable material that shows excellent mechanical properties, diverse applications, biocompatibility and biodegradability. Transgenic silkworm technology was used to obtain four types of chimeric silkworm/spider (termed composite) silk fibres, including different lengths of recombinant Major ampullate Spidroin1 (re-MaSp1) or recombinant Major ampullate Spidroin2 (re-MaSp2) from the black widow spider,
Latrodectus hesperus
. The results showed that the overall mechanical properties of composite silk fibres improved as the re-MaSp1 chain length increased, and there were significant linear relationships between the mechanical properties and the re-MaSp1 chain length (p |
| doi_str_mv | 10.1038/s41598-018-34150-y |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6206087</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2127203177</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-5daa7541d5ddef41ab9ec3263ddcd489d40f33179948fd85ea1151932b6b528f3</originalsourceid><addsrcrecordid>eNp9kctu1DAUhiMEaqvSF-gCWWLDJsXXxNkgVaOBIrUC0enacuKTjIfEDnZSOg_BO9fTKaWwwBsf6XznP5c_y04JPiOYyfeRE1HJHBOZsxTifPsiO6KYi5wySl8-iw-zkxg3OD1BK06qg-yQYVZyTKuj7NfybgraB2OdDlt0Bc1aO9voHn0NfoQwWYjIt2jhh9FHOwG6tv13tFoHP3drdAEBjJ103QNaBe1iB6n6gfnpw4CWd2OAGK3r0Ddo_FCnNm5CV3rjAzofxrnv9U5ztCZ4615nr1rdRzh5_I-zm4_L1eIiv_zy6fPi_DJvBCFTLozWpeDECGOg5UTXFTSMFsyYxnBZGY5bxkhZVVy2RgrQhAhSMVoXtaCyZcfZh73uONcDmAZcOkKvxmCHdAXltVV_Z5xdq87fqoLiAssyCbx7FAj-xwxxUoONDaRtHPg5KkpoSXEaYYe-_Qfd-Dm4tN6OKqRMRvFE0T3VBB9jgPZpGILVznC1N1wlw9WD4Wqbit48X-Op5Le9CWB7IKaU6yD86f0f2XtarLrH</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2126882044</pqid></control><display><type>article</type><title>Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin</title><source>Publicly Available Content Database</source><source>Full-Text Journals in Chemistry (Open access)</source><source>PubMed Central</source><source>Springer Nature - nature.com Journals - Fully Open Access</source><creator>You, Zhengying ; Ye, Xiaogang ; Ye, Lupeng ; Qian, Qiujie ; Wu, Meiyu ; Song, Jia ; Che, Jiaqian ; Zhong, Boxiong</creator><creatorcontrib>You, Zhengying ; Ye, Xiaogang ; Ye, Lupeng ; Qian, Qiujie ; Wu, Meiyu ; Song, Jia ; Che, Jiaqian ; Zhong, Boxiong</creatorcontrib><description>Spider dragline silk is a remarkable material that shows excellent mechanical properties, diverse applications, biocompatibility and biodegradability. Transgenic silkworm technology was used to obtain four types of chimeric silkworm/spider (termed composite) silk fibres, including different lengths of recombinant Major ampullate Spidroin1 (re-MaSp1) or recombinant Major ampullate Spidroin2 (re-MaSp2) from the black widow spider,
Latrodectus hesperus
. The results showed that the overall mechanical properties of composite silk fibres improved as the re-MaSp1 chain length increased, and there were significant linear relationships between the mechanical properties and the re-MaSp1 chain length (p < 0.01). Additionally, a stronger tensile strength was observed for the composite silk fibres that included re-MaSp1, which only contained one type of repetitive motif, (GA)
n
/A
n
, to provide tensile strength, compared with the silk fibres that includedre-MaSp2, which has the same protein chain length as re-MaSp1 but contains multiple types of repetitive motifs, GPGXX and (GA)
n
/A
n
. Therefore, the results indicated that the nature of various repetitive motifs in the primary structure played an important role in imparting excellent mechanical properties to the protein-based silk fibres. A silk protein with a single type of repetitive motif and sufficiently long chains was determined to be an additional indispensable factor. Thus, this study forms a foundation for designing and optimizing the structure of re-silk protein using a heterologous expression system.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-34150-y</identifier><identifier>PMID: 30374029</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>101/28 ; 14 ; 14/1 ; 38 ; 38/1 ; 38/44 ; 38/77 ; 42 ; 42/109 ; 42/44 ; 631/337 ; 631/61 ; Amino acid sequence ; Animals ; Animals, Genetically Modified ; Biocompatibility ; Biodegradability ; Biodegradation ; Black Widow Spider - genetics ; Bombyx - genetics ; Bombyx - metabolism ; Chromosomes, Artificial, Bacterial ; Exons - genetics ; Fibers ; Fibroins - chemistry ; Fibroins - genetics ; Genes, Insect ; Genes, Synthetic ; Genetic Vectors ; Genotype ; Humanities and Social Sciences ; Latrodectus hesperus ; MASP-1 protein ; MASP-2 protein ; Mechanical properties ; Microscopy, Electron, Scanning ; multidisciplinary ; Protein structure ; Proteins ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - chemistry ; Repetitive Sequences, Amino Acid ; Science ; Science (multidisciplinary) ; Silk ; Silk - chemistry ; Silk - ultrastructure ; Spiders ; Stress, Mechanical ; Tensile Strength</subject><ispartof>Scientific reports, 2018-10, Vol.8 (1), p.15956-14, Article 15956</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-5daa7541d5ddef41ab9ec3263ddcd489d40f33179948fd85ea1151932b6b528f3</citedby><cites>FETCH-LOGICAL-c511t-5daa7541d5ddef41ab9ec3263ddcd489d40f33179948fd85ea1151932b6b528f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2126882044/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2126882044?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,25734,27905,27906,36993,36994,44571,53772,53774,74875</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30374029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>You, Zhengying</creatorcontrib><creatorcontrib>Ye, Xiaogang</creatorcontrib><creatorcontrib>Ye, Lupeng</creatorcontrib><creatorcontrib>Qian, Qiujie</creatorcontrib><creatorcontrib>Wu, Meiyu</creatorcontrib><creatorcontrib>Song, Jia</creatorcontrib><creatorcontrib>Che, Jiaqian</creatorcontrib><creatorcontrib>Zhong, Boxiong</creatorcontrib><title>Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Spider dragline silk is a remarkable material that shows excellent mechanical properties, diverse applications, biocompatibility and biodegradability. Transgenic silkworm technology was used to obtain four types of chimeric silkworm/spider (termed composite) silk fibres, including different lengths of recombinant Major ampullate Spidroin1 (re-MaSp1) or recombinant Major ampullate Spidroin2 (re-MaSp2) from the black widow spider,
Latrodectus hesperus
. The results showed that the overall mechanical properties of composite silk fibres improved as the re-MaSp1 chain length increased, and there were significant linear relationships between the mechanical properties and the re-MaSp1 chain length (p < 0.01). Additionally, a stronger tensile strength was observed for the composite silk fibres that included re-MaSp1, which only contained one type of repetitive motif, (GA)
n
/A
n
, to provide tensile strength, compared with the silk fibres that includedre-MaSp2, which has the same protein chain length as re-MaSp1 but contains multiple types of repetitive motifs, GPGXX and (GA)
n
/A
n
. Therefore, the results indicated that the nature of various repetitive motifs in the primary structure played an important role in imparting excellent mechanical properties to the protein-based silk fibres. A silk protein with a single type of repetitive motif and sufficiently long chains was determined to be an additional indispensable factor. Thus, this study forms a foundation for designing and optimizing the structure of re-silk protein using a heterologous expression system.</description><subject>101/28</subject><subject>14</subject><subject>14/1</subject><subject>38</subject><subject>38/1</subject><subject>38/44</subject><subject>38/77</subject><subject>42</subject><subject>42/109</subject><subject>42/44</subject><subject>631/337</subject><subject>631/61</subject><subject>Amino acid sequence</subject><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Biocompatibility</subject><subject>Biodegradability</subject><subject>Biodegradation</subject><subject>Black Widow Spider - genetics</subject><subject>Bombyx - genetics</subject><subject>Bombyx - metabolism</subject><subject>Chromosomes, Artificial, Bacterial</subject><subject>Exons - genetics</subject><subject>Fibers</subject><subject>Fibroins - chemistry</subject><subject>Fibroins - genetics</subject><subject>Genes, Insect</subject><subject>Genes, Synthetic</subject><subject>Genetic Vectors</subject><subject>Genotype</subject><subject>Humanities and Social Sciences</subject><subject>Latrodectus hesperus</subject><subject>MASP-1 protein</subject><subject>MASP-2 protein</subject><subject>Mechanical properties</subject><subject>Microscopy, Electron, Scanning</subject><subject>multidisciplinary</subject><subject>Protein structure</subject><subject>Proteins</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - chemistry</subject><subject>Repetitive Sequences, Amino Acid</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Silk</subject><subject>Silk - chemistry</subject><subject>Silk - ultrastructure</subject><subject>Spiders</subject><subject>Stress, Mechanical</subject><subject>Tensile Strength</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNp9kctu1DAUhiMEaqvSF-gCWWLDJsXXxNkgVaOBIrUC0enacuKTjIfEDnZSOg_BO9fTKaWwwBsf6XznP5c_y04JPiOYyfeRE1HJHBOZsxTifPsiO6KYi5wySl8-iw-zkxg3OD1BK06qg-yQYVZyTKuj7NfybgraB2OdDlt0Bc1aO9voHn0NfoQwWYjIt2jhh9FHOwG6tv13tFoHP3drdAEBjJ103QNaBe1iB6n6gfnpw4CWd2OAGK3r0Ddo_FCnNm5CV3rjAzofxrnv9U5ztCZ4615nr1rdRzh5_I-zm4_L1eIiv_zy6fPi_DJvBCFTLozWpeDECGOg5UTXFTSMFsyYxnBZGY5bxkhZVVy2RgrQhAhSMVoXtaCyZcfZh73uONcDmAZcOkKvxmCHdAXltVV_Z5xdq87fqoLiAssyCbx7FAj-xwxxUoONDaRtHPg5KkpoSXEaYYe-_Qfd-Dm4tN6OKqRMRvFE0T3VBB9jgPZpGILVznC1N1wlw9WD4Wqbit48X-Op5Le9CWB7IKaU6yD86f0f2XtarLrH</recordid><startdate>20181029</startdate><enddate>20181029</enddate><creator>You, Zhengying</creator><creator>Ye, Xiaogang</creator><creator>Ye, Lupeng</creator><creator>Qian, Qiujie</creator><creator>Wu, Meiyu</creator><creator>Song, Jia</creator><creator>Che, Jiaqian</creator><creator>Zhong, Boxiong</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20181029</creationdate><title>Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin</title><author>You, Zhengying ; Ye, Xiaogang ; Ye, Lupeng ; Qian, Qiujie ; Wu, Meiyu ; Song, Jia ; Che, Jiaqian ; Zhong, Boxiong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-5daa7541d5ddef41ab9ec3263ddcd489d40f33179948fd85ea1151932b6b528f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>101/28</topic><topic>14</topic><topic>14/1</topic><topic>38</topic><topic>38/1</topic><topic>38/44</topic><topic>38/77</topic><topic>42</topic><topic>42/109</topic><topic>42/44</topic><topic>631/337</topic><topic>631/61</topic><topic>Amino acid sequence</topic><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Biocompatibility</topic><topic>Biodegradability</topic><topic>Biodegradation</topic><topic>Black Widow Spider - genetics</topic><topic>Bombyx - genetics</topic><topic>Bombyx - metabolism</topic><topic>Chromosomes, Artificial, Bacterial</topic><topic>Exons - genetics</topic><topic>Fibers</topic><topic>Fibroins - chemistry</topic><topic>Fibroins - genetics</topic><topic>Genes, Insect</topic><topic>Genes, Synthetic</topic><topic>Genetic Vectors</topic><topic>Genotype</topic><topic>Humanities and Social Sciences</topic><topic>Latrodectus hesperus</topic><topic>MASP-1 protein</topic><topic>MASP-2 protein</topic><topic>Mechanical properties</topic><topic>Microscopy, Electron, Scanning</topic><topic>multidisciplinary</topic><topic>Protein structure</topic><topic>Proteins</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - chemistry</topic><topic>Repetitive Sequences, Amino Acid</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Silk</topic><topic>Silk - chemistry</topic><topic>Silk - ultrastructure</topic><topic>Spiders</topic><topic>Stress, Mechanical</topic><topic>Tensile Strength</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>You, Zhengying</creatorcontrib><creatorcontrib>Ye, Xiaogang</creatorcontrib><creatorcontrib>Ye, Lupeng</creatorcontrib><creatorcontrib>Qian, Qiujie</creatorcontrib><creatorcontrib>Wu, Meiyu</creatorcontrib><creatorcontrib>Song, Jia</creatorcontrib><creatorcontrib>Che, Jiaqian</creatorcontrib><creatorcontrib>Zhong, Boxiong</creatorcontrib><collection>SpringerOpen</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>You, Zhengying</au><au>Ye, Xiaogang</au><au>Ye, Lupeng</au><au>Qian, Qiujie</au><au>Wu, Meiyu</au><au>Song, Jia</au><au>Che, Jiaqian</au><au>Zhong, Boxiong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-10-29</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>15956</spage><epage>14</epage><pages>15956-14</pages><artnum>15956</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Spider dragline silk is a remarkable material that shows excellent mechanical properties, diverse applications, biocompatibility and biodegradability. Transgenic silkworm technology was used to obtain four types of chimeric silkworm/spider (termed composite) silk fibres, including different lengths of recombinant Major ampullate Spidroin1 (re-MaSp1) or recombinant Major ampullate Spidroin2 (re-MaSp2) from the black widow spider,
Latrodectus hesperus
. The results showed that the overall mechanical properties of composite silk fibres improved as the re-MaSp1 chain length increased, and there were significant linear relationships between the mechanical properties and the re-MaSp1 chain length (p < 0.01). Additionally, a stronger tensile strength was observed for the composite silk fibres that included re-MaSp1, which only contained one type of repetitive motif, (GA)
n
/A
n
, to provide tensile strength, compared with the silk fibres that includedre-MaSp2, which has the same protein chain length as re-MaSp1 but contains multiple types of repetitive motifs, GPGXX and (GA)
n
/A
n
. Therefore, the results indicated that the nature of various repetitive motifs in the primary structure played an important role in imparting excellent mechanical properties to the protein-based silk fibres. A silk protein with a single type of repetitive motif and sufficiently long chains was determined to be an additional indispensable factor. Thus, this study forms a foundation for designing and optimizing the structure of re-silk protein using a heterologous expression system.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30374029</pmid><doi>10.1038/s41598-018-34150-y</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2018-10, Vol.8 (1), p.15956-14, Article 15956 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6206087 |
| source | Publicly Available Content Database; Full-Text Journals in Chemistry (Open access); PubMed Central; Springer Nature - nature.com Journals - Fully Open Access |
| subjects | 101/28 14 14/1 38 38/1 38/44 38/77 42 42/109 42/44 631/337 631/61 Amino acid sequence Animals Animals, Genetically Modified Biocompatibility Biodegradability Biodegradation Black Widow Spider - genetics Bombyx - genetics Bombyx - metabolism Chromosomes, Artificial, Bacterial Exons - genetics Fibers Fibroins - chemistry Fibroins - genetics Genes, Insect Genes, Synthetic Genetic Vectors Genotype Humanities and Social Sciences Latrodectus hesperus MASP-1 protein MASP-2 protein Mechanical properties Microscopy, Electron, Scanning multidisciplinary Protein structure Proteins Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Repetitive Sequences, Amino Acid Science Science (multidisciplinary) Silk Silk - chemistry Silk - ultrastructure Spiders Stress, Mechanical Tensile Strength |
| title | Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T14%3A37%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Extraordinary%20Mechanical%20Properties%20of%20Composite%20Silk%20Through%20Hereditable%20Transgenic%20Silkworm%20Expressing%20Recombinant%20Major%20Ampullate%20Spidroin&rft.jtitle=Scientific%20reports&rft.au=You,%20Zhengying&rft.date=2018-10-29&rft.volume=8&rft.issue=1&rft.spage=15956&rft.epage=14&rft.pages=15956-14&rft.artnum=15956&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-018-34150-y&rft_dat=%3Cproquest_pubme%3E2127203177%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c511t-5daa7541d5ddef41ab9ec3263ddcd489d40f33179948fd85ea1151932b6b528f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2126882044&rft_id=info:pmid/30374029&rfr_iscdi=true |